Synthesis and Biocompatibility Assessment of a Cysteinebased Nanocomposite for Applications in Bone Tissue Engineering

다국어 초록 (Multilingual Abstract)

Purpose To develop a robust ECM and enhance cellularmaterialinteraction, we developed a cysteine-based nanocompositecombined with chitosan, nanohydroxyapatite andgelatin (cysteine-CnHAG) to effectuate essential factors.
Methods The scaffolds were fabricated under same conditionsusing a freeze-drying technique and characterized with ascanning electron microscopy to study its internal architectureincluding pore distribution. They were further interactedwith human bone marrow derived mesenchymal stem cells(hMSCs) to investigate its biocompatibility. In this, cell viabilityand cell adhesion was assessed using optical microscopy.
Results From the results, the scaffolds display a non-toxiceffect with hMSCs and have a pore size range of between12-142 μm. The result of the optical microscopy showedadhesion of the cells on the scaffold matrix of both cysteine-CnHAG and CnHAG, which implies the microenvironmentof the scaffold is largely suitable for the MSC developmentConclusions In conclusion, we believe cysteine-CnHAG canbe a novel nanocomposite for potential applications in thefield of bone tissue engineering.

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