Ginsenoside Rg3 및 그 유도체 Ginsenoside Rg3-2H의 NO 생성 및 lymphocyte 분열 억제 효과

다국어 초록 (Multilingual Abstract)

Ginsenosides are major components in Panax ginseng and known to have numerous pharmacological activities such as anti-cancer, anti-diabetes, anti-viral and anti-atherosclerosis effects. In this study, the regulatory activities of G-Rg3 and its derivative 25-hydroxy Rg3 (G-Rg3-2H) on the production of nitric oxide (NO) in macrophages and the proliferation of lymphocytes prepared from spleen and bone marrow under treatment of lipopolysaccharide (LPS) or concanavalin (Con) A were examined. G-Rg3 and G-Rg3-2H dose-dependently inhibited NO production from LPS-activated RAW264.7 cells and in agreement, these compounds protected RAW264.7 cells from LPS-mediated cytotoxicity. In contrast, G-Rg3-2H dose-dependently inhibited lymphocyte proliferation induced by both LPS and Con A, while there was no inhibition by G-Rg3. Therefore, our data suggest that these compounds may be applied for NO-mediated or lymphocyte- mediated immunological diseases.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 결론
• 감사의 글
• 인용문헌

참고문헌 (Reference)

1 Olszewski W. L., "The lymphatic system in body homeostasis:physiological conditions" 1 : 11-21, 2003

2 Rivera E., "The Rb1 fraction of ginseng elicits a balanced Th1 and Th2 immune response" 23 : 5411-5419, 2005

3 Campbell J. D., "T cell chemokine receptor expression in human Th1- and Th2-associated diseases" 48 : 451-456, 2000

4 Rice A. P., "Regulation of TAK/P-TEFb in CD4+ T lymphocytes and macrophages" 1 : 395-404, 2003

5 Gillis C. N., "Panax ginseng pharmacology: a nitric oxide link?" 54 : 1-8, 1997

6 Sternberg E. M., "Neuroendocrine regulation of autoimmune/inflammatory disease" 169 : 429-435, 2001

7 Tetley T. D., "Inflammatory cells and chronic obstructive pulmonary disease" 4 : 607-618, 2005

8 Cho J. Y., "In vitro inhibitory effect of protopanaxadiol ginsenosides on tumor necrosis factor (TNF)-alpha production and its modulation by known TNF-alpha antagonists" 67 : 213-218, 2001

9 Cho J. Y., "In vitro anti-inflammatory effects of cynaropicrin, a sesquiterpene lactone, from Saussurea lappa" 398 : 399-407, 2000

10 Sun J. L., "Immunologic enhancement of compound Chinese herbal medicinal ingredients and their efficacy comparison with compound Chinese herbal medicines" 24 : 2343-2348, 2006

11 Cho J. Y., "Ginsenosides from Panax ginseng differentially regulate lymphocyte proliferation" 68 : 497-500, 2002

12 Szabo C., "DNA damage induced by peroxynitrite: subsequent biological effects" 1 : 373-385, 1997

13 Palmer R. M., "Chronic sialadenitis. An immunocytochemical study in humans" 412 : 73-78, 1987

14 Kim B. H., "Anti-inflammatory effect of honokiol is mediated by PI3K/Akt pathway suppression" 29 : 113-122, 2008