有鉤襄尾蟲의 鉤와 表皮의 形態에 關한 硏究 = Morphology of Cysticercus cellulosae Rostellar Hooks and Epidermis Found on Cheju Island

국문 초록 (Abstract)

本 硏究는 囊尾蟲의 分類에 必要한 有鉤囊尾蟲의 形態를 밝히고저 額嘴에 있는 鉤數, 크기, 形態 및 囊尾蟲의 頭節部, 頸部, 吸盤에 있는 絨毛를 光學顯微鏡과 走査電子顯微鏡을 利用하여 觀察하였다.
囊尾蟲에 感染된 雌雄돼지 및 屠畜場에서 囊蟲에 感染된 屠肉으로부터 囊尾蟲을 採集하여 利用하였다. 또 人體의 囊尾蟲 및 有鉤條蟲의 額嘴에 있는 鉤數, 形態, 크기를 觀察하였다.

1. 雌雄돼지에서 檢出된 囊尾蟲의 鉤크기는, 長鉤는 共히 136∼187㎛範圍이며, 平均 148.0 ±1.3㎛였다. 또 短鉤에 있어서는 68∼153㎛範圍이었고 平均120 ±1.4㎛였다.
雄豚에 寄生한 囊尾蟲은 雌豚의 것보다 長鉤가 많고, 奇生部位別로 보면 耳內 및 四肢筋에 奇生하는 囊尾蟲에 큰 鉤가 있었다. 또 腦에 奇生하는 囊尾蟲에는 제일 작은 鉤를 볼 수 있었다. 雌雄豚의 어는 것이나 長鉤나 短鉤의 크기는 囊尾蟲의 奇生部位가 다름으로써 平均値間에 高度의 有意差가 있었다 (P＜0.01).

2. 雌雄豚에서 檢出된 囊尾蟲의 鉤數는 雌豚은 平均 23.6 ±0.4 였다. 또 제일 頻度가 많은 26 個의 鉤를 가진 個體는 全體의 32.7%였다. 또 雄豚에 있어서는 鉤數가 平均25.2 ±0.6이었지만, 26個의 鉤를 가지고 있는 것은 43.3%이었다. 그것들의 囊尾蟲 個體에 따른 鉤數는 偶數것이 奇數것 보다 많았다. 囊尾蟲의 鉤數 平均値는 部位別로 有意差가 있었다 (P＜0.05).

3. 사람의 皮下 또는 筋肉에서 檢出된 囊尾蟲의 長鉤 크기는 平均 167.8 ±0.9㎛이며, 그 分布는 170㎛것이 71.4%, 153㎛것이 16.4%였다. 또 短鉤는 平均 114.1 ±1.0㎛이며 119㎛것이 55.4%, 102㎛것이 30.2%를 占하고 있었다.
鉤數는 平均 27.9個이며 28個것이 全體의 70.0%를 占하고 있었다.

4. 有鉤條蟲의 額嘴에 있는 長短鉤의 크기와 數는, 長鉤가 平均 153.4 ±0.7㎛이였고, 153㎛의 鉤가 92.5%로 제일 많았다. 또 短鉤는 平均 124.2 ±1.7㎛이고, 119㎛것이 53.8%로 제일 많았다.

5. 돼지 囊尾蟲의 走査電子顯微鏡的所見에서는 額嘴에 있는 鉤의 handle과 guard는 完全히 額嘴內에 박혀 있었다. blade가 額嘴外에 露出되어 있는 部分은 象牙狀이고 上下 2列로 大小鉤가 配列되어 있었다. 또 特히 頭節部 및 頸部와 吸盤部에는 特有의 絨毛狀構造로된 乳頭가 密生되어 있는 것을 確認하였다.

다국어 초록 (Multilingual Abstract)

Studies were made to positively identify Cysticercus cellulosae based on morphology. Microvilli in scolex, sucker and neck, and rostellar hook humbers, size and shape, were examined in detail using optical microscopic and electron scanning microscopic techniques. Samples were taken from male and female pigs infected with C. cellulosae and taken from similarly infected pork at an abattoir. Observations were also made of rostellar hook numbers, shape and size in C. cellulosae hominis and Taenia solium.
1. Rostellar hook sizes of the larger hook group expelled from male and female pigs were in the range of 136-187㎛ with a mean of 148.0 ±1.3㎛ while in the smaller hook group the hook sizes ranged from 68 to 153㎛ with a mean of 120.4 ±1.4㎛ Statistically significant differences (p＜0.01) in hook sizes were observed depending not only on male and female but also on the infected sites; males had larger hooks than females and the larger hooks were found in ears and/or extremity muscle while the smaller hooks were found in the brain.
2. The female pig's average hook number was 23.6 ±0.4 and 32.7% of the total had 26 hooks. In the male pigs, however, the mean hook number was 25.2 ±0.6 and 43.3% of the total has also 26 hooks. It was observed that even numbers of rostellar hooks were greater than odd numbers.
Significant differences(p＜0.05) in the mean numbers of rostellar hook were found depending on the infected sites : this tendency was very similar to that of hook size.
3. The hook sizes of C,. cellulosae homonis obtained from subcutenous tissue or muscle of human had a mean of 167.8 ±0.9㎛(71.4%)followed by a measurment of 153㎛(16.4%). The smaller hook group had a mean value of 114.1 ±10㎛and was composed of hook sizes of 119㎛(55.4%) and 102㎛(30.2%). Average number of hooks was 27.9 and 70% of the total had 28 hooks in each C. cellulosae hominis.
4. The rostellar hook size and number of Taenia solium had a mean value of 153.4 ±0.7㎛ in the larger hook group and the greatest number (92.5%) was observed in the 153㎛size. In the smaller hook group the average size was 124.2 ±1.7㎛ and 53.8% were 119㎛.
5. Scanning electronic microscopus observation showed that the handle and guard of the rostellar hook was completely concealed by rostellium but the blade only partly. The blades appeared like a very small ivory tusk pointing outwards and double alternating large and small hooks.
A dense distribution of microvilli was observed in scolex, sucker and rostellum of C. cellulosae.