Streptozotocin 유발성 당뇨백서에서 해당효소에 대한 Phlorizin의 효과 = Effect of Phlorizin on Glycolytic Enzymes in Streptozotocin-Diabetic Rat

다국어 초록 (Multilingual Abstract)

In glycolysis, the reactions catalyzed by hexokinase, Phosphofructokinase, and pyruvate kinase are lally irreversible; hence they would be expected to have regulatory as well as catalytic roles. They ilso adaptive in a number of physiological states, and although the molecular mechanisms controlling adaptations are largely unknown, dietary and hormonal stimuli serve as environmental factors lating enzyme levels in vivo.
The present study was undertaken to examine the effects of phlorizin, a blocker of renal tubular ose reabsorption, on the activities of glucokinase, hexokinase, and pyruvate kinase in the liver and nrocytes of streptozotocin (STZ) diabetic rats.
The levels of plasma glucose and insulin and the activities of glycolytic enzymes were measured onchird day after phlonzin treatment in normal and STZ diabetic rats and the results were as follows.
1. Plasma glucose levels were reduced by 14.35%(P＜0.05) in normal rats and by 39.38% (p＜0.01) in STZ diabetic rats.
2. Plasma insulin levels were reduced by 45.6% (P＜0.05) in normal rats and by 58.2% (P＜0.01) in STZ diabetic rats.
3. On the third day after phlorizin treatment, hepatic glucokinase and pyruvate kinase activities in normal rats were reduced by approximately 56% (P＜0.01) and 43% (P＜0.01) respectively, and erythrocyte hexokinase and pyruvate kinase activities were reduced by approximately 18% (P＜0.05) and 22% (P＜0.01) respectively, In STZ diabetic rat shepatic glucokinase and pyruvate kina activities were reduced by approximately 52% (P＜0.01) and 22% (P<0.01) respectively, a erythrocyte hexokmase and pyruvate kinase activities were reduced by approximately 4% and 3 respectively.
Thus the overall decreases of the levels of plasma glucose and insulin and the activity of glycoly enzymes were observed on the third day after phlorizin treatment in normal and STZ diabetic ra These alterations in the enzyme activity with phlorizin treatment were shown to be a result of reducinsulin levels, and the smaller decrease in enzyme actvity in STZ diabetic rats than in normal rats thought to be probably due to the reduced response to changes in insulin levels at near basal levels enzyme activity.