Bacillus sp. N-25가 생산하는 Xylanase의 특성 = Characterization of a Xylanase Produced by Bacillus sp. N-25

국문 초록 (Abstract)

섬유소 자원의 효율적 이용을 위하여 강력한 xylan 분해효소 생산 세균을 분리 및 선발하였으며, 형태학적, 배양학적 생리학적 특성을 확인한 결과 Bacillus sp.N25 로 동정하였다.
이 균주에 의해서 생산된 효소를 ammonium sulfate precipitation, DEAE-sephadex A-50, Sephadex G-100 column으로 부분 정제하여 얻은 효소의 열안정성은 50℃에서 30분간 처리시 80%의 역가가 잔존했으며, pH 안정은 pH 6∼B 범위에서 30℃에서 10시간 방치 후에도 안정했으며, cellulose 분해능력은 없었으며, Hg^2+, Ag^2+, Mn^2+에 의해 저해되었다. 그리고 최종분해산물은 주로 xylose이므로 exo-type xylanase로 추정된다.

다국어 초록 (Multilingual Abstract)

To increase the efficency of utilizing cellulosic biomass, a potent xylanase producing bacteria was isolated and identified as Bacillus sp. N-25. Extracellular xylanase from Bacillus sp. N-25 was partially purified by ammonium sulfate precipitation, DEAE-Sephadex A-25 and Sephadex G-100 column chromatographies. The xylanase was single fraction on chromatography and was true xylanase without cellulase activity. The enzyme was stable at pH 6∼8 and 80% activity was remained at 50℃ for 30 min, but was inhibited by Hg^2+, Ag^2+ and Mn^2+. From the fact that the major end product was xylose, we suggested that the enzyme is an exo-xylanase which may be a prime candidate for industrial use.