쥐 배종양세포의 Endo-B 유전자 발현 = Studies on Gene Expression of Endo-B in Murine Embryonal Carcinoma Cells

다국어 초록 (Multilingual Abstract)

Endo-B, the mouse form of Keratin 18, is the first member of the large intermediate filament gene family to be expressed during embryogenesis. Thus, the differentiation of cultured murine embryonal carcinoma cells has been used as a convenient model system of early mouse development. F9,22(mouse embryonal carcinoma) cells were treated with retinoic acid (5 x 10_6M/l) for 72 hours, and then they were fixed with the mixture of glutaraldehyde(2%) and formaldehyde(2%) in phosphate buffered saline for 5 min at 4℃. The fixed cells were immunoreacted with the rabbit anti-Endo-B antiserum. Endo-B was visualized by reaction with rhodamine conjugated goat anti-rabbit antibodies. Some of the differentiated F9 cells exhibited strong fluorescence indicating the expression of Endo-B filament.