High-throughput Screening Strategy Used for Enhanced Production of Pigment by Monascus purpureus D39-4

다국어 초록 (Multilingual Abstract)

Most of the fermentation experiment designs were limited by the low-throughput of shake flask, especially for the medium optimization. A simple high-throughput screening system was developed for the determination of pigment in Monascus purpureus fermentation samples.
This downscaled system was designed to optimize medium composition combined with statistical methods. The total 29 experiments designed by the Box–Behnken were used to study the 4 most important operating variables on pigment production. The analysis revealed that the optimum concentrations of glucose, peptone, NaNO3, and KH2PO4were 51.42, 4.91, 1.00, and 1.00 g/L, respectively. A production of 69.5 U/mL was achieved in agreement with the prediction (68.9 U/mL) fermented in 24-deep-well microtiterplates. Furthermore, the fermentation medium optimized in the high-throughput system was verified in shake flasks, and the pigment production could be enhanced from 206.5 U/mL in un-optimized medium to 265.8 U/mL,giving nearly 1.30-fold increase in production.

참고문헌 (Reference)

1 He YQ, "Use of response surface methodology to optimize culture medium for production of lipase with Candida sp" 43 : 9-14, 2006

2 Isett K, "Twenty-four well plate miniature bioreactor high-throughput system: Assessment for microbial cultivations" 98 : 1017-1028, 2007

3 Carels M, "The effect of different nitrogen sources on pigment production and sporulation of Monascus sp. in submerged shaken culture" 23 : 1360-1372, 1977

4 Kim SS, "Studies on production and characteristics of edible red color pigment produced by mold" 11 : 277-283, 1977

5 Pujari V, "Statistical optimization of medium components for enhanced riboflavin production by a UV-mutant of Eremothecium ashbyii" 36 : 31-37, 2000

6 Kaushik R, "Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus" 40 : 121-126, 2006

7 Kapdan IK, "Simultaneous biodegradation and adsorption of textile dyestuff in an activated sludge unit" 37 : 973-981, 2002

8 Elibol M, "Response surface analysis of lipase production by freely suspended Rhizopus arrhizus" 38 : 367-372, 2002

9 Blanc PJ, "Production of citrinin by various species of Monascus" 17 : 291-294, 1995

10 Shimizu T, "Polyketide synthase gene responsible for citrinin biosynthesis in Monascus purpureus" 71 : 3453-3457, 2005

11 Pisareva E, "Pigments and citrinin biosynthesis by fungi belonging to genus Monascus" 60 : 115-120, 2005

12 Botz DW, "Parallel reactor systems for bioprocess development" 92 : 125-143, 2005

13 Liu CH, "Optimizing lipase production of Burkholderia sp. by response surface methodology" 41 : 1940-1944, 2006

14 Sharma P, "Optimization of process variables for decolorization of disperse Yellow 211 by Bacillus subtilis using Box-Behnken design" 164 : 1024-1029, 2009

15 Gao YL, "Optimization of process conditions to inactivate Bacillus subtilis by high hydrostatic pressure and mild heat using response surface methodology" 24 : 43-48, 2005

16 Xiong ZQ, "Optimization of medium composition for actinomycin X2 production by Streptomyces spp. JAU4234 using response surface methodology" 35 : 729-734, 2008

17 Deepak V, "Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology" 99 : 8170-8174, 2008

18 Gong GL, "Mutation and a HTS method for improving the production of Epothilones of Sorangium" 34 : 615-623, 2007

19 Chagas GM, "Mechanism of citrinin-induced dysfunction of mitochondria. 2. Effect onrespiration, enzyme-activities, and membrane-potential of livermitochondria" 10 : 209-216, 1992

20 Ruchi G, "Lipase from solvent tolerant Pseudomonas aeruginosa strain: Production optimization by response surface methodology and application" 99 : 4796-4802, 2008

21 Gao H, "Identification of avermectin-high-producing strains by HTS methods" 85 : 1219-1225, 2010

22 Huang L, "HTS of high-yield colonies of Rhizopus oryzae for enhanced production of fumaric acid" 60 : 287-292, 2010

23 Wang D, "Effects of oils and oil-related substrates on the synthetic activity of membrane bound lipase from Rhizopus chinensis and optimization of the lipase fermentation media" 41 : 30-37, 2008

24 Krejci ME, "Citrinin produces acute adverse changes in renal function and ultrastructure in pentobarbitalanesthetized dogs without concomitant reductions in [potassium] plasma" 106 : 167-177, 1996

25 Blanc PJ, "Characterization of monascidin-A from Monascus as citrinin" 27 : 201-213, 1995

26 Betts JI, "Characterization and application of a miniature 10 mL stirred-tank bioreactor, showing scale-down equivalence with a conventional 7 L reactor" 22 : 681-688, 2006

27 Ji Yeun Kim, "Characteristics of Monascus sp. Isolated from Monascus Fermentation Products" 한국식품과학회 19 (19): 1151-1157, 2010

28 Shao P, "Analysis of immobilized Candida rugosa lipase catalyzed preparation of biodiesel from rapeseed soapstock" 86 : 283-289, 2008

29 Li ZQ, "A further studies on the species of Monascus" 23 : 1-6, 2004