중금속 이온 존재 하에서의 아스코르브산 자동산화반응에 대한 단백질의 영향 = Effect of various proteins on the autoxidation rates of L-Ascorbic acid in the presence of heavy metal ions

다국어 초록 (Multilingual Abstract)

Effects of superoxide dismutase(SOD), catalase, and such other proteins as bovine serum albumin(BSA), ovalbumin, lysozyme, and γ-globulin on the autoxidation rates of L-ascorbic acid(AsA) in the presence of heavy metal ions in aqueous solution and buffer solution were studied. AsA was dissolved in a ultra-refined water and 0.07 M potassium phosphate buffer (pH 7.4, treated Chelex-100) at a concentration of 50μM and 5 μM Fe(Ⅲ) or 0.1 μM Cu(Ⅱ) was added, and a oxygen gas was bubbled through the solution at a flow rate of 200 ml/min at 35℃. The amount of remaining AsA in the reaction mixture was determined by using a UV spectrophotometer(at 265 nm). It was confirmed that the ratio of remaining AsA was significantly larger in the presence of SOD, catalase, BSA, ovalbumin, lysozyme, and γ-globulin than in the absence of proteins. It was suggested that the non-enzymatic effects of SOD, catalase and some other proteins might be involves in the stabilization of AsA.