순비기나무(Vitex rotundifolia) 줄기 추출물의 폴리페놀 함량과 생리활성 = Polyphenol Compound Contents and Physiological Activities in Various Extracts of the Vitex rotundifolia Stems

국문 초록 (Abstract)

순비기나무(Vitex rotundifolia)의 줄기를 새로운 천연 항산화제나 생리활성 재료로 이용하기 위하여 한류 물 추출물(WE)과 에탄을 추출물(EE) 그리고 열수 추출물(HWE)에 대하여 총 폴리페놀 함량과 전자공여능, SOD 유사활성능, 아질산염 소거능 및 xanthine oxidase와 tyrosinase 저해 활성을 측정하였다. 총 폴리페놀의 함량을 측정한 결과 122.01 mg/g${\sim}$176.34 mg/g으로 EE에서 가장 많은 폴리페놀을 함유하였다. 전자공여능은 1.0 mg/mL의 농도에서 93.46${\sim}$96.92%로 EE가 가장 높았으며 0.1 mg/mL에서도 90% 이상이었으며, SOD 유사활성능은 WE가 47.32%로 가장 높은 활성을 나타내었다. 아질산염 소거능은 pH 1.2의 조건에서 세가지 추출물이 84.61${\sim}$88.36%였으며 pH 3.0에서는 25.83${\sim}$30.24%였다. Xanthine oxidase 저해 활성을 측정한 결과 0.5 mg/mL의 농도에서 세 가지 추출물 모두 90% 이상이었으며, 1.0 mg/mL에서도 91.02${\sim}$97.51%로 EE 추출물이 가장 높은 저해 활성을 나타내었다. 미백효과와 관련이 있는 tyrosinase 저해 활성을 측정한 결과 HWE와 WE는 57.84%와 53.47%를 나타내어 EE(28.79%)보다 높은 저해 효과가 나타났다. 따라서 순비기나무 줄기 추출물은 다량의 폴리페놀을 함유하며 항산화 활성과 xanthine oxidase 및 tyrosinase 저해 활성이 우수하므로 천연 항산화제나 미백 및 기능성 식품소재로써 활용할 수 있을 것으로 기대된다.

다국어 초록 (Multilingual Abstract)

For this study, extracts of Vitex rotundifolia stems were prepared using reflux water extraction (WE), reflux ethanol extraction (EE) and hot water extract under high pressure (HWE). The extracts were investigated for the total content of phenolic compounds, antioxidant activities, and inhibitory potencies for xanthine oxidase and tyrosinase. The EE extraction method yielded the highest content of polyphenol compounds (176.34 mg/g). The electron donating abilities (EDA) were 93.46${\sim}$96.92%, when extracts were assayed at 1.0 mg/mL. The superoxide dismutase (SOD)-like activity was the highest in the WE extract (47.32% at 1.0 mg/mL). The nitrite scavenging abilities (pH 1.2) were 84.61${\sim}$88.36% and the inhibition of xanthine oxidase were over 90% at 0.5 mg/mL. Tyrosinase inhibition of HWE and WE were 57.84% and 53.47% respectively. It implies that V. rotundifolia stems have potent physiological activities and their activities were differently exhibited depending on solvent fractions.

참고문헌 (Reference)

1 김은영, "항산화활성을 나타내는 약용식물 소재 탐색" 한국식품과학회 36 (36): 333-338, 2004

2 임정대, "한국산 약용식물로부터의 SOD 활성 및 Phenolic Compounds 함량 비교" 한국약용작물학회 12 (12): 191-202, 2004

3 김선재, "약용식품 추출에 대한 항산화성과 알코올 탈수소효소 저해성 연구" 한국식품저장유통학회 11 (11): 201-206, 2004

4 정성제, "약용 식물 추출물의 항산화 활성 검색" 한국응용생명화학회 47 (47): 135-140, 2004

5 김남우, "싸리(Lespedeza bicolor) 추출물의 항산화성에 관한 연구" 한국식품저장유통학회 12 (12): 75-79, 2005

6 김남우, "싸리 추출물의 폴리페놀 함량과 생리활성" 한국식품저장유통학회 13 (13): 616-622, 2006

7 장수정, "순비기나무(Vitex rotundifolia L. fil.)의 부위별 정유성분 조성" 한국응용생명화학회 45 (45): 101-107, 2002

8 허준, "동의보감 한글완역본" 대중서관 293-1445, 1994

9 강명화, "각 부위별 활나물(Crotalaria sessiflora L.) 에탄올 추출물의 항산화효과" 한국식품과학회 34 (34): 1098-1102, 2002

10 國家中医药管理局編委会, "中華本草, Vol 6" 上海科學技術出版社 604-608, 1999

11 Sanchez-Ferer A, "Tyrosinase a comprehensive review of its mechanism" 1247 : 1-11, 1995

12 Shin DH, "The study course and movement of natural antioxidants" 30 : 14-18, 1997

13 Stirpe F, "The regulation of rat liver xanthine oxidase" 244 : 3855-3861, 1969

14 Kramer HM, "The association between gout and nephrolithiasis" 40 : 37-42, 2002

15 Kim SM, "The antioxidant ability and nitrate scavenging ability of plant extract" 33 : 623-632, 2001

16 Jung SW, Lee NK, Kim SJ, Han DW, "Screening of tyrosinase inhibitor from plants" 27 : 891-896, 1995

17 Watanabe K, "Rotundial, a new natural mosquito repellent from the leaves of Vitex rotundifolia" 59 : 1979-1980, 1995

18 Ko WG, "Polymethoxyflavonoids from Vitex rotundifolia inhibit proliferation by inducing apoptosis in human myeloid leukemia cells" 38 : 861-865, 2000

19 Kang SS, "Phytochemical analysis of Viticis Fructus" 25 : 214-220, 1994

20 Yoshioka T, "Phenolic compounds and flavonoids as plant growth regulators from fruit and leaf of Vitex rotundifolia" 59 : 509-514, 2004

21 Okuyama E, "Pharmacologically active components of Vitis Fructus(Vitex rotundifolia). Ⅱ. The components having analgesic effects" 46 : 655-662, 1998

22 McCord JM, "Oxygen-derived radicals; a link between repercussion injury and inflammation" 46 : 2402-2406, 1987

23 AOAC., "Official Method of Analysis. 18th ed. Vol 45" Association of Official Analytical Chemists 21-22, 2005

24 Fiala ES, "Naturally occurring anticarcinogenic substances in foodstuffs" 5 : 295-321, 1985

25 Pratt DE., "Natural antioxidants from plant materials, In Phenolic Compounds in Food and Their Effects on Health (Ⅱ)" Am Chem Soc 54-60, 1992

26 Shin KH, "Isolation of an aldose reductase inhibitor from the fruits of Vitex rotundifolia" 1 : 46-48, 1994

27 Marklund S, "Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase" 47 : 468-474, 1975

28 But PPH, "International collation of traditional and folk medicine" World Scientific Publishing 141-142, 1996

29 Moon SH, "Inhibitory effects of xanthine oxidase by boiled water extract and tannin from persimmon leaves" 11 : 354-357, 1998

30 Hashiguchi H, "Inhibition of two copper containing enzymes, tyrosinase and dopamine β-hydroxylase, by L-mimosine" 13 : 362-367, 1976

31 Kato H, "Inhibition of nitrosamine formation by nondialyzable melanoidins" 51 : 1333-1338, 1987

32 Yagi A, "Inhibition of mushroom-tyrosinase by aloe extract" 53 : 517-519, 1987

33 Leaf CD, "Influence of ascorbic acid dose on N-nitrosoproline formation in humans" 8 : 791-795, 1987

34 Lee TB., "Illustrated Flora of Korea. 5th ed" Hyangmoonsa 644-, 1993

35 Yeeh Y, "Genetic and clonal diversity in Korean populations of Vitex rotundifolia(Verbenaceae)" 109 : 161-168, 1996

36 Kobayakawa J, "G2-M arrest and antimitotic activity mediated by casticine, a flavonoid isolated from Viticis Fructus(Vitex rotundifolia Linne fil. )" 208 : 59-64, 2004

37 Mirvish SS, "Formation of N-nitroso compounds : chemistry, kinetics, and in vivo occurrence" 31 : 325-351, 1975

38 Kwon TD, "Effects of polyphenol or vitamin C ingestion on antioxidative activity during exercise in rats" 3 : 891-899, 2001

39 Shin TY, "Effect of Vitex rotundifolia on immediate-type allergic reaction" 72 : 443-450, 2000

40 Ames BN, "Dietary carcinogens and anticarcinogens. Oxygen radical and degenerative disease" 221 : 1256-1264, 1983

41 Akaike T, "Determination of peroxyl radical-scavenging activity in food by using bactericidal action of alkyl peroxyl radical" 43 : 1864-1870, 1995

42 Hatano T, "Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols" 49 : 357-363, 1995

43 Freeman BA, "Biology of disease; free radicals and tissue injury" 47 : 412-426, 1982

44 Kim HK, "Antioxidative activity and physiological activity of some Korean medical plants" 27 : 80-85, 1995

45 Blois MS, "Antioxidant determination by the use of a stable free radical" 181 : 1199-1200, 1958

46 Miyazawa M, "Antimutagenic activity of(+)-polyalthic acid from Vitex rotundifolia" 43 : 3012-3015, 1995