Adenovirus증식의 초기과정

다국어 초록 (Multilingual Abstract)

Approximately 30separate adenovisuses have been isolated from man. Visuses in this group replicate in the nuclei of infected cells and are of similar size(60-90 nm in diameter) and shape. All mernbers appear to have a core composed of single molecule of DNA and common compliment fixing structure visus antigen. Adenovirsus infection in man produce acute pharygitis, tracheo bronchitis, bronchopneumonia or conjunctivitis. Several members of this group, including types commonly associated with respiratory disease in man, are capable of producing malignant tumors in young hamsters, and a few types have been shown to be oncogenic in young rats. Adenoviruses types 1, 2 and 5 have been recovered from surgically removed tonsil and adenoids and have been also associated with sporadic mild respiratory illness of infant and children. To obtain evidence concerning the early period of multiplication of adenoviruses, investigation of 4 proto type viruses of this group was undertaken. For practical consideration it was desirable to investigate of the adsorption and initial cycle of multiplication. The experimental results indicate that the adenoviruses studied adsorb at a relatively slow rate to Hela cell; that the latent or eclipse period of the multiplication cycle is relatively long and is longer for type 1 and 2 than for type 4 and 5; that the incremental period of viral propagation is relatively short. Results were as follows : 1. Following combination of virus with Hela cell(direct measurement of adenovirus adsorption), maximun virus adsorption required 6-9 hours. 2. Result of development of cytopathic effects in cells exposed to virus for varing periods (indirect measurement of adenovirus adsorption) indicate that adenovirus type 1 and type 5 were adsorbed faster than type 2 and type 4. 3. The latent or eclipse period of the multiplication cycle is relatively long and is longer for type 1 and 2 than for type 4 and 5. The latent period for type 1 and 2 viruses were approximately 18 hours, whereas for the related type 4 and 5 viruses the latent periods were significantly shorter, lasting about 12-13 hours. 4. The incrernental period of viral propagation is relatively short. Incremental period for type 1 and 2 viruses was 10-12 hours. For the type 4 and 5 viruses, howver, incremental period was somewhat longer.