식염수 및 증류수의 간세포에 미치는 영향에 관한 전자현미경적 연구 = An Electron Microscopic Studies of Effects of Sodium Chloride Solution and Distilled Water on Rats Liver Cells

다국어 초록 (Multilingual Abstract)

In sodium chloride intoxication, many authors have reported that it may cause various degree and combination of regressive changes and hemorrhagic disorders on different organs in the living body, of which the most pronounced alteration may occur in the liver.
However, there is no report of electron microscopic study to determine the relationships between the structural changes and functional alterations as yet.
Therefore, in an effort of further clarification of the structural changes and these relationships, the author breeded some albino rats with 2％ and 4％ sodium chloride solution and distilled water as usual drinking water, and then the animals were sacrified on the first, second and fourth weeks respectively, from which the liver were extracted out and studied under both light and electron microscopes; the following results were obtained:
1. The body weight was decreased in both groups fed with sodium chloride solution and distilled water, but in was more prominent in the former group; the more serious weight loss was observed in the group to which more prolonged administration of sodium chloride was given.
2. In the group fed on the distilled water, hydrophic swelling and mild degree of destructive changes in the liver cells were observed light microscopically and in addition, mitotic figures were also noted in many cases.
3. in the group fed on sodium chloride solution, the boundaries among hepatocytes were obscure because of flaky appearence of cytoplasm and various degree of cellular destruction, and the mitotic figures could not be observed light microscopically in any cases. In the group on 2％ and 4％ sodium chloride solution, the shrin kage and destructive changes of hepatocytes were observed.
4. Mild dilatation of rough-surfaced endoplasmic reticulum in the group fed on distilled water, and prominent swelling, deformation, distortion of cristae and decreased electron opacity of matrix in the mitochondria, increase of lysosomes and smooth-surfaced endoplasmic reticulum, and decrease of glycogen particles in the group on 2％ and 4％ sodium chloride have been noted electron microscopically.In addition, focal cytoplasmic degradation and doughnut-shaped structures were also detected. These changes have a tendency to increase in degree in accordance with the period of administration and concentration of sodium chloride solution.
5. It is suggest that the mitochondrial degeneration and the increase of smooth-surfaced endoplasmic reticulum have a relationship with the imbalance of intracellular electrolytes, and the decrease of glycogen particles with the proliferation of smooth-surfaced endoplasmic reticulum.
6. The appearance of focal cytoplasmic degradation and increase of lysosomes, judging from their forms, seem to have relationship with the phenomenon known as autophagocytosis, and this could be interpreted as one way of manifestation of degenerative changes of cells.
7. The author also observed a doughnut-shaped structure, a intracellular abnormal structure which was first reported by Steiner and Phillips. However, based upon the result of this experiment only, it is still obscure whether this has direct relationship with over administration of sodium chloride solution or not.