벼 흰잎마름병균(Xanthomonas campestris pv. oryzae)의 形質轉換體에 관한 연구 = Transformants between races of Xanthomonas campestis pv. oryzae

다국어 초록 (Multilingual Abstract)

Transformation between races of Xanthomonas campestris pv. oryzae was attempted to illustrate possible occurrance of new races or strains of greater virulence than their parental strains. An auxotrophic mutant(JB206A-1(race K1)) requiring either methionine or cystein, and a white colony mutant(JB 8206W-1(race K1))were transformed to prototroph and yellow colony formation respectively, by treating with DNA of HB83216(race K2) or JN8041(race K3). On the average, transformants showed increased virulence to rice cultivar Milyang 23 as compared with their recipient strains. Three transformants of JB8206A-1 treated with DNA of JN8041 were virulent to rice cultivar Cheongcheong as well as Milyang 23. The standard deviations of lesion lengths on the differential cultivars were greater for transformants than for respective parental strains, suggesting that transformants were genetically more heterogeneous than their parental strains. Bacteriological characteristics of transformants were generally similar to those of their parental strains. However, Two transformants of JB8206W-1 exhibited positive reaction in nitrate reduction although their parental strains showed negative reaction.