We constucted two expression vetors, pSSK-1 and pSSK-6, carrying IL-2 gene for the purpose of estimating the amount of its products and the effects in the potato. The pSSK-6 is under the control of 35S CaMV promoter(pSSK-1) specifically expressed in p...
We constucted two expression vetors, pSSK-1 and pSSK-6, carrying IL-2 gene for the purpose of estimating the amount of its products and the effects in the potato. The pSSK-6 is under the control of 35S CaMV promoter(pSSK-1) specifically expressed in potato tuber. Also, pSSK-1, a binary vector carrying interleukin-2 (IL-2) gene, was constructed to be examine the possibility of the production of IL-2 and its effects in potato, and transferred into A. tumefaciens for the sake of the transformation of potato (solanum tuberosum cv. Superior).
In our recent work, we verified that IL-2 gene from pSSK-1 vector was expressed in all parts of the transformed potato plant. However the amount of IL-2 protein was estimated very little.