Propagation of Lymphocystis disease virus (LCDV) in the FFN cell Line originated from olive flounder Paralichthys olivaceus fin = Propagation of Lymphocystis disease virus (LCDV) in the FFN cell Line originated from olive flounder Paralichthys olivaceus fin

다국어 초록 (Multilingual Abstract)

The present study demonstrated lymphocystis disease virus (LCDV) propagation through cytopathic effects (CPE) formation and LCDV detection in olive flounder fin (FFN) cells by polymerase chain reaction (PCR) and fluorescent antibody technique (FAT) methods. Tissue filtrates from the cluster cells produced CPE in FFN cells, which initially cells became enlarged and gradually underwent fusion en masse. Infectivity of culture grown LCDV using the FFN cells reached 10(2.3) TCID50/ml at 4 days post infection and the highest titer was measured 10(6.5) TCID50/ml at 12 days. The viral DNA was detected in the cell culture supernatants showing CPE and the CPE cells by PCR. Antigen specific strong fluorescence reacting with monoclonal antibody against the virus revealed the presence of viral antigen in the cytoplasm of infected FFN cells. These results suggest that the FFN cell line originated from the olive flounder has a susceptibility of the LCDV.

참고문헌 (Reference)

1 Zwillenberg, L.D, "Ultrastructure of lymphocystis virus" 2 : 393-399, 1968

2 Tidona, C.A, "The complete DNA sequence of lymphocystis disease virus" 230 : 207-216, 1997

3 Walker, D.P, "Studies on the culture assay of infectivity and some in vitro properties of lymphocystis virus" 51 : 385-395, 1980

4 Nigrelli, R.F, "Studies on lymphocystis disease in the orange filefish, Ceratacanthus schoepfi (Walbaum) from Sandy Hook Bay, New Jersey" 24 : 255-264, 1939

5 Wolf, K, "Lymphocystis virus: isolation and propagation in centrarchid fish cell lines" 151 : 1004-1005, 1966

6 Hossain, M, "Lymphocystis disease virus persists in the epidermal tissues of olive flounder, Paralichthys olivaceus (Temminch & Schlegel), at low temperatures" 32 : 699-703, 2009

7 Wolf, K, "Lymphocystis disease in Fish viruses and fish viral diseases" Cornell University Press, Ithaca, New York, USA 268-291, 1988

8 Garcia-Rosado, E, "Isolation and characterization of lymphocystis virus (FLDV) from gilt-head sea bream (Sparus aurata, L.) using a new homologous cell line" 19 : 53-56, 1999

9 Schnitzler, P, "Identification of the gene encoding the major capsid protein of fish lymphocystis disease virus" 74 : 2143-2150, 1993

10 Lannan, C.N, "Fish cell lines: establishment and characterization of nine cell lines from salmonids" 20 : 671-676, 1984

11 Walker, R, "Fine structure of lymphocystis disease virus of fish" 18 : 503-505, 1962

12 Chinchar, V.G, "Family Iridoviridae in Virus taxonomy: Eighth report of the international committee on taxonomy of viruses" Elsevier/Academic Press, London United Kingdom 145-162, 2005

13 Kasai, H, "Establishment of 2 Japanese flounder embryo cell lines" 52 : 67-70, 2001

14 Kang, M.S, "Establishment and characterization of two new cell lines derived from flounder, Paralichthys olivaceus (Temminck & Schlegel)" 26 : 657-665, 2003

15 Shin-Ichi Kitamura, "Differentiation of Lymphocystis Disease Virus Genotype by Multiplex PCR" 한국미생물학회 44 (44): 248-253, 2006

16 Cano, I, "Development of molecular techniques for detection of lymphocystis disease virus in different marine fish species" 102 : 32-40, 2006

17 Cano, I, "Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head sea bream (Sparus aurata L.) using an immunoblot technique" 113 : 137-141, 2006

18 Zhang, Q.Y, "Complete genome sequence of lymphocystis disease virus isolated from China" 78 : 6982-6994, 2004

19 Samalecos, C.P, "Analysis of the structure of fish lymphocystis disease virions from skin tumors of Pleuronectes" 91 : 1-10, 1986

20 Reed, L.J, "A simple method of estimating fifty percent endpoint" 27 : 493-497, 1938

21 Gravel, M, "A permanent cell line from the fathead minnow (Pimephales promelas)" 126 : 555-565, 1965

22 Kitamura, S.I, "A new genotype of lymphocystivirus, LCDV-RF, from lymphocystis diseased rockfish" SPRINGER-VERLAG WIEN 151 (151): 607-615, 2006