Effects of titanium oxide nanoparticles on Oryzias latipes embryos and sac-fry under different irradiation conditions

다국어 초록 (Multilingual Abstract)

Some phototoxicity of titanium dioxide nanoparticles (TiO₂ NPs) has been reported in recent years in studies with fish embryos or larvae. However, it is necessary to focus on the potential effects of embryonic exposure due to irreversible abnormalities and mortalities observed in sac-fry, and to expand various fish embryos to generate multiple test species. The aim of this study was to evaluate the effects of TiO₂ NPs under different irradiation conditions in exposed Oryzias latipes (O. latipes) at the embryonic and sac-fry stages. The effects of different irradiation conditions were observed using ultra-violet (UV) and visible light, and the corresponding effects were monitored by determining cumulative mortality and abnormality. O. latipes were exposed for 8 d to 0, 1, 5, 10, or 50 mg/L TiO₂ NPs under UV (4,818.86 mW/m² at the bottom of clear vials) or visible light, after which the embryos were transferred to NP-free embryo-rearing solution until 16 days post fertilization (dpf). Abnormalities of embryos and sac-fry increased at high TiO₂ NP concentrations under UV irradiation, compared to control samples treated with visible light or UV irradiation alone. This work provides information regarding the phototoxicity of TiO₂ NPs using O. latipes at the embryonic and sac-fry stages.

목차 (Table of Contents)

• ABSTRACT
• 1. Introduction
• 2. Materials and Methods
• 3. Results and Discussion
• 4. Conclusions
• References