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    RAPD-PCR을 이용한 마우스 계통간 유연관계 = GENETIC RELATONSHIP AMONG THE MOUSE STRAINS USING RAPD-PCR

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    https://www.riss.kr/link?id=A19599676

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    다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

    We have analyzed the genetic relationship among the mouse strains using RAPD-PCR. Polymorphic RAPD markers amplified from various primers were used to analyze the genetic relationship among seven mouse strains, CBA, C57BL/6, BALB/C, NOD, A/wy, DBA2, and AKR. The genetic similarity coefficients were estimated using the RAPD markers by UPGMA method. The genetic similarity coefficient between CBA and NOD was 0.474. This value was lower than any other genetic similarity coefficient among the strains tested. And CBA-specific RAPD band patterns were observed in 5 primers from 6 polymorphic primers used. This study makes possible phylogenetic identification among the inbred mouse strains at the molecular level using RAPD-PCR techniques.
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    We have analyzed the genetic relationship among the mouse strains using RAPD-PCR. Polymorphic RAPD markers amplified from various primers were used to analyze the genetic relationship among seven mouse strains, CBA, C57BL/6, BALB/C, NOD, A/wy, DBA2, a...

    We have analyzed the genetic relationship among the mouse strains using RAPD-PCR. Polymorphic RAPD markers amplified from various primers were used to analyze the genetic relationship among seven mouse strains, CBA, C57BL/6, BALB/C, NOD, A/wy, DBA2, and AKR. The genetic similarity coefficients were estimated using the RAPD markers by UPGMA method. The genetic similarity coefficient between CBA and NOD was 0.474. This value was lower than any other genetic similarity coefficient among the strains tested. And CBA-specific RAPD band patterns were observed in 5 primers from 6 polymorphic primers used. This study makes possible phylogenetic identification among the inbred mouse strains at the molecular level using RAPD-PCR techniques.

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    목차 (Table of Contents)

    • I. 서 론
    • Ⅱ. 재료 및 방법
    • 1. 마우스 계통
    • 2. Genomic DNA 분리
    • 3. Primer와 PCR
    • I. 서 론
    • Ⅱ. 재료 및 방법
    • 1. 마우스 계통
    • 2. Genomic DNA 분리
    • 3. Primer와 PCR
    • 4. 전기 영동
    • 6. 계통간의 유전적 유연관계분석
    • Ⅲ. 결과 및 고찰
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