고열충격반응에 영향을 미치는 초파리 hsp70 유전자의 DNA 염기배열 = DNA Sequences of the Drosophila melanogaster hsp70 Gene Affecting Heat Shock Responses

다국어 초록 (Multilingual Abstract)

The DNA sequences required for heat shock regulated expression were examined in Drosophila melanogaster. Various hsp70-YP1 fusion constructs where D. melanogaster hsp70 gene and YP1 gene were used as a heat shock promoter and as a target gene, respectively, were transfected into D. melanogaster Schneider line 2 cells and their heat inducibility was determined by Northern analysis. The sequences containing two HSEs between -89 and -38 of the hsp70 were not sufficient to induce YP1 RNA upon heat shock whereas the sequences between -89 and +34 including the TATA box and the transcription start region of the hsp70 besides the HSEs were able to drive YP1 transcription during heat shock. The small deletion of the sequences near the transcription start region of the YP1 gene was also observed to repress the heat induced transcription of YPl RNA by the sequences between -89 and +34 of the hsp70.