RBL-2H3에서 IgE-dependent Histamine-releasing Factor에 의한 활성산소종 생성에 관한 연구 = Generation of ROS by IgE-Dependent Histamine-Releasing Factor in PBL-2H3 Cells

다국어 초록 (Multilingual Abstract)

Histamine-releasing factors (HRFs) are soluble mediators that can release histamine and other mediators from basophils and mast cells and their activity can vary, depending on the type of IgE. The activity of HRFs is affected by the presence of IgE, although HRF is thought to bind to a specific receptor other than IgE. Until now, HRF signaling pathway including its receptor its receptor remains unclear in spite of numerous studies. Since there had been many reports about reactive exygen species (ROS) as a signaling molecule rather than as a by-product of metabolism, we investigated the possibility of ROS as an intracellular messenger involved in HRF-mediated histamine degranulation. In RBL-2H3 cells, ROS was generated by HRF using H₂O₂-sensitive fluorescence of fluorescent 2', 7'-dichlorofluorescein H₂DCFDA). There effects were blocked by anti-oxidant N-acetylcysteine (NAC). These results suggest that ROS generation could play a role as an intracellular messenger in histamine release by HRF.

목차 (Table of Contents)

• 요약
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