Screening and Cloning of RAPD Markers from the W Chromosome of Silkworm, Bombyx mori L.

다국어 초록 (Multilingual Abstract)

Silkworms sex determination drew high attention
from researchers. Sex chromosomes on the silkworm
are of ZW type for females and ZZ type for males.
Chromosome W plays an important role in sex determination.
Although several molecular linkage maps
have been constructed for silkworm, very few markers
are discovered on the W chromosome. In order to look
for molecular markers and to further locate the Fem
gene on chromosome W, we used genomic DNA from
both female and male larvae of a silkworm strain
named 937 as PCR templates for RAPD amplification
with 200 arbitrary 10-mer primers. The amplification
results showed three female-specific bands, namely
OPG-07496, OPC-151,660 and OPE-181,279. Further verification,
however, revealed no band from OPG-07 and
OPC-15 in either sex in the strain 798, but OPE-18
provided female-specific band in the strains Suluan7
and C108, and absent in both males and strain 798.
This indicates that the bands from OPG-07496 and
OPC-151,660 are probably female-specific in strain 937,
and the band from OPE-18 was probably amplified
from a common segment shared by most strains. The
genomic DNAs from OPG-07 and OPC-15 were cloned
and sequenced. Sequence analysis showed that the
DNAs from OPG-07 and OPC-15 have high identities
with the retrotransposable elements, and DNA from
OPC-15 contains a portion of sequence which probably
encodes an eukaryotic translation initiation factor
4E binding protein (eIF4EBP).

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