Six new dammarane-type triterpene saponins from Panax ginseng flower buds and their cytotoxicity

다국어 초록 (Multilingual Abstract)

Background: Panax ginseng has been used for a variety of medical purposes in eastern countries formore than two thousand years. From the extensive experiences accumulated in its long medication usehistory and the substantial strong evidence in modern research studies, we know that ginseng hasvarious pharmacological activities, such as antitumor, antidiabetic, antioxidant, and cardiovascularsystemeprotective effects. The active chemical constituents of ginseng, ginsenosides, are rich instructural diversity and exhibit a wide range of biological activities.
Methods: Ginsenoside constituents from P. ginseng flower buds were isolated and purified by variouschromatographic methods, and their structures were identified by spectroscopic analysis and comparisonwith the reported data. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H- tetrazolium bromidemethod was used to test their cytotoxic effects on three human cancer cell lines.
Results: Six ginsenosides, namely 6’emalonyl formyl ginsenoside F1 (1), 3beacetoxyl ginsenoside F1 (2),ginsenoside Rh24 (6), ginsenoside Rh25 (7), 7behydroxyl ginsenoside Rd (8) and ginsenoside Rh26 (10)were isolated and elucidated as new compounds, together with four known compounds (3e5 and 9). Inaddition, the cytotoxicity of these isolated compounds was shown as half inhibitory concentration values,a tentative structureeactivity relationship was also discussed based on the results of our bioassay.
Conclusion: The study of chemical constituents was useful for the quality control of P. ginseng flowerbuds. The study on antitumor activities showed that new Compound 1 exhibited moderate cytotoxicactivities against HL-60, MGC80-3 and Hep-G2 with half inhibitory concentration values of 16.74, 29.51and 20.48 mM, respectively.

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