To enhance the xylitol production in xylose-fermenting yeast Pichia stipitis, an expression vector was constructed to constitutively express the xylose reductase gene in P. stipitis. While the control P. stipitis strain produced 40.6 ± 0.9 g ethanol ...
To enhance the xylitol production in xylose-fermenting yeast Pichia stipitis, an expression vector was constructed to constitutively express the xylose reductase gene in P. stipitis. While the control P. stipitis strain produced 40.6 ± 0.9 g ethanol from 100 g xylose in aerobic shake flasks cultivation, without forming xylitol, P. stipitis MBY1381, which constitutively expresses the xylose reductase gene by the vector, produced 34.9 ± 0.9 g xylitol utilizing 40 g xylose, corresponding to an approximate yield of 87%.