때죽나무(Styrax japonica) 수피 추출물의 항암 활성 = Anti-cancer Activity of Styrax japonica Bark Extrats

다국어 초록 (Multilingual Abstract)

A compound has been isolated from the methanol extract of Styrax japonica bark using conventional chromatographic methods including silica gel chromatography, TLC and HPLC. The molecular formula of Styraxlignolide F analyzed by spectrometric analyses using FAB-MS, NMR was found to be C27H34O11Na. The cytotoxicity of the styralignolide F was showed 15.2% in 1.0 ㎎/㎖ on human kidney cell (HEK 293). As anticancer activity of CH2Cl2 fraction, over 60% of AGS and MCF-7 cells were inhibited in concentration of 1.0 ㎎/㎖. In the results of anticancer test using quantification of Bcl-2, CH2Cl2 fraction showed lower Bcl-2 and p53 expression than those of styraxlignolide F and other fractions. In apoptosis of human lung carcunoma cancer cell (A549), CH2Cl2 fraction showed the highest inhibition rate (46.9%) and styralignolide F was the next (43.5%). The CH2Cl2 fraction showed higher anti-cancer activ ities than isolated substance (styraxlignolide F), probably due to the crude extract showing synergic effects by other components.

참고문헌 (Reference)

1 Lopes., 22 : 1516-1518, 1983

2 한상섭, "산림식물을 이용한 약제, 건강음료 및 분재용 수목 개발에 관한 연구" 26-, 2000

3 권오웅, "때죽나무의 부위별 면역 및 항암활성 비교" 한국약용작물학회 15 (15): 170-176, 2007

4 정우택, "감초 세근의 생리활성 탐색" 한국약용작물학회 9 (9): 45-54, 2001

5 Vardar, V., "Preliminary studies on the Styrax oil" 22 : 145-151, 1973

6 이태진, "Hep 3B 간암세포주에서 Mycolactone이 Bcl-2 Family 유전자들의 발현에 미치는 영향" 대한미생물학회 32 (32): 55-61, 2002

7 May A., "Glycolaldehyde induces apoptosis in a human breast cancer cell line" 417 (417): 123-127, 2003

8 Davis, P. H., "Flora of Turkey and the East Aegean Islands, vol, 4" University of Edinburgh Press 432-, 1972

9 Costa, A. F., "Farmacognosia, second ed., vol, 1" Fundacao Calouste Gulbenkian 737-, 1968

10 Pratima Sur., "Estrogen attenuates oxidative stress-induced apoptosis in C6 glial cells" 971 : 178-188, 2003

11 Haldar S., "Down-regulation of bcl-2 by p53 in breast cancer cells" 54 : 2095-2097, 1994

12 Dlyle A., "Cell & Tissue Culture, Laboratory Procedures" Wiley 1993

13 Frebourg T., "Cancer risks from germline p53 mutation" 91 : 1637-1641, 1992

14 Reed JC., "Bcl-2 family proteins : regulators of cell death involved in the pathogenesis of cancer and resistance to therapy" 60 : 23-32, 1996

15 Reed JC., "Bcl-2 and the regulation of programmed cell death" 124 : 1-6, 1994

16 Sinicrope FA., "Bcl-2 and p53 oncoprotein expression during colorectal tumorigenesis" 55 : 237-241, 1995