통증 억제에 이용된 크로마핀 세포가 분비하는 Catecholamine 생체 내외 정량분석 = Quantitative Analysis of Secreted Catecholamines from Chromaffin Cells in Vitro and in Vivo

다국어 초록 (Multilingual Abstract)

Background: Adrenal medullary transplants into the subarachnoid space have been demonstrated to reduce pain sensitivity. This analgesia most likely results from the release of neuroactive substances, particularly catecholamines and opioid peptides from the transplanted cells into spinal cord.
Methods: Isolated bovine chromaffin cells were encapsulated with alginate and poly-L-lysine prior to implantation into rat’s subarachnoid space to protect them from host immune system. And then catecholamines from encapsulated chromaffin cells were measured quantitatively in vitro by High Performance Liquid Chromatograph. The animals were randomized into 2 groups, one of which received microencapsulated chromaffin cells and the other empty capsules. The effects of such implants were evaluated on the pain behavior resulting from a chronic constriction injury of the rat sciatic nerve for 30 days.
Results: Catecholamine concentration in cerebrospinal fluid (CSF) was analyzed. Data (mean SD) are considered significant at P ＜ 0.05 (ANOVA for repeated measure and Dunnett's test). Continuous release of catecholamine and met-enkephalin with responsiveness to nicotine stimulation was measured from encapsulated cells in vitro. A significant reduction of allodynic response to acetone evaporation was observed in the animals implanted with cell loaded capsules compared to control animals with empty capsules. Catecholamine concentration in CSF was higher in the cell loaded capsule group. There were no complications related to implantation.
Conclusions: We found that encapsulated chromaffin cells released continuously catehcolamines and opioids peptides in vitro and in the CSF. Those results may prove chromaffin cell's anagesic effect indirectly.

국문 초록 (Abstract)

저자들은 통증모델 쥐에서 채취한 뇌척수액으로 APA (alginate/poly-L-lysine/alginate) 삼중 반 투과막 캡슐로 캡슐화한 크로마핀 세포를 자극하여 catecholamines이 분비되는 것을 시험관에서 관찰하였다. 또한 통증모델 쥐에 이식 후 쥐 뇌척수액 내의 catecholamines양이 이식 전에 비해 2배 이상 증가하는 것을 관찰함으로써 이식된 캡슐화된 크로마핀 세포에서 분비된 catecholamines이 신경병증성 통증모델 쥐에서 이질통에 대한 반응 억제효과를 나타내는 주요한 물질 중의 하나임을 확인하였다.

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