더덕과 일부 한약재 열수추출물의 혼합 비율에 따른 생리활성 = Physiological Functionalities of Hot Water Extract of Codonopsis lanceolata and Some Medicinal Materials, and Their Mixtures

다국어 초록 (Multilingual Abstract)

The aim of this study was to investigate the physiological functionalities of Codonopsis lanceolata, Glycyrrhiza uralensis, Chaenomeles sinensis, Crataegus pinnafida, and their mixtures. We also determined their antioxidative, fibrinolytic, and αglucosidase inhibitory activities. The antioxidative activities of Codonopsis lanceolata, Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida were 79%, 88.3%, 89.9%, and 89.3% respectively. Their fibrinolytic activities were 0.80plasmin unit/ml, 0.57 plasmin unit/ml, 0.52 plasmin unit/ml, and 0.53 plasmin unit/ml respectively. The αglucosidase inhibitory activity of Codonopsis lanceolata was 25%. The 10fold diluents of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida showed αglucosidase inhibitory activities of 93.6%, 65.3%, and 61.3% respectively. In antioxidative activity tests of the medicinal plants mixtures at various ratios, the mixtures of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida with Codonopsis lanceolata showed antioxidative activities of approximately 90%. In fibrinolytic activity tests mixtures(1:1) of Codonopsis lanceolata with Chaenomeles sinensis and Crataegus pinnafida exhibited increases of 23% and 24% in activity respectively. In αglucosidase inhibitory activity tests, a mixture (4:1) of Codonopsis lanceolata and 10fold diluted Glycyrrhiza uralensis showed an inhibitory activity of 98%, a mixture (3:1) of Codonopsis lanceolata and 10fold diluted Chaenomeles sinensis showed an inhibitory activity of 69.6%, and a mixture (1:1) of Codonopsis lanceolata and 10fold diluted Crataegus pinnafida showed an inhibitory activity of 50.2%. In conclusion, the mixtures of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida with Codonopsis lanceolata will be used as a material for the development of biofunctional foods.

참고문헌 (Reference)

1 "한약재 열수추출물의 항산화효과 검정" 43 (43): 141-147, 2000

2 "청국장으로부터 혈전용해균주의 분리 및 동정" 23 : 1-5, 1995

3 "천연 항산화제의 연구동향과 방향" 30 (30): 14-, 1997

4 "약용식품으로 한약재의 이용" 5 (5): 50-56, 2000

5 "된장에서 분리한 Bacillus amyloliquefaciens가 분비하는 혈전용 해효소 활성의 양상" 5 : 139-145, 1998

6 "김치의 혈전용해작용" 5 : 203-210, 1995

7 "and the degenerative diseases of aging" 1933

8 "alpha-Glucosidase inhibitors from the seeds of Balsam Pear (Momordica charantia) and the fruit bodies of Grifola frondosa" 66 : 1576-1578, 2002

9 "Triterpenoids from Codonopsis lanceolata" 79-84, 1976

10 "Technology Trends in Functional Foods" 36 (36): 17-, 2003

11 "Studies on the terpenoid component of the roots of Codonopsis lanceolata Benth et Hook" 8 : 49-53, 1997

12 "Rapid purification and biochemical characteristics of Lumbrokinase Ⅲ from earthworm for use as a fibrinolytic agent" 20 : 169-172, 1998

13 "Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. Strain CK 11-4 screened from ChungkookJang" 2482-2488, 1996

14 "Plant regeneration and saponin contents in Condonopsis lanceolata L" 7 : 275-281, 1999

15 "Pharmacologe and chemical constituents of the root of Codonopsis pilosula Nannfeldt" 8 : 43-48, 1997

16 "Pharmacognostical Studies on Codonpsis Iancelata" 43-47, 1975

17 "Partial purification and characterization of fibrinolytic substance from Wooltalkong (Phaseolus ssp.)" 10 : 415-420, 2004

18 "Market Trends in Functional Foods" 36 (36): 8-, 2003

19 "Isolation of antioxidative components of Perillae emen" 29 : 38-43, 1997

20 "Isolation and identification of alpha-glucosidase inhibitors from Tochu-cha (Eucommia ulmoides)" 61 : 177-178, 1997

21 "Isoflavone contents, antioxidative and fibrinolytic activities of some commercial cooking-with-rice soybeans" 34 : 498-504, 2002

22 "Isoflavone contents, antioxidative and fibrinolytic activities of red bean and mung bean" 19 : 263-270, 2003

23 "Inhibitory effects of Proanthocyanidin extracted from Distylium racemosum on α-amylase and α-glucosidase activities" 35 (35): 271-275, 2004

24 "In vitro Inhibitory activity on rat intestinal Mucosa α-Glucosidase by rice hull extract" 29 (29): 601-608, 1997

25 "Fibrinolytic and cogulation activities of korean snake venoms" 696-701, 1992

26 "Dose-response curves in the fibrin plate assay Fibrinolytic activity of protease" 356-365, 1974

27 "Development of functional soy-based stew sauce including hot water extract of Cornus officinalis S. et Z" 21 (21): 550-558, 2006

28 "Chemical Sudies on the Alkaloidal Constituents of Codonopsis lanceolata" 1-7, 1986

29 "Characterization of potent fibrinolytic enzymes in earthworm" 1993

30 "Characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish" 84 : 307-312, 1997

31 "Change of fibrinolytic activities of Condonopsis lanceolata according to various storage conditions, and heat or salt treatments" 11 : 63-69, 2005

32 "Antioxidant determinations by the use of a stale free radical" 1199-181 1120, 1958

33 "A fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea" 63 : 2130-2136, 1999