세포병리검사에서 쓸개관암종의 IMP3 발현과 진단적 가치 = IMP3 Expression of the Cholangiocarcinoma in Cytology Specimen and Its Diagnostic Value

다국어 초록 (Multilingual Abstract)

Background : Pathologists occasionally have difficulty in distinguishing cholangiocarcinoma from benign reactive cells on the biliary cytology. We estimated the diagnostic value of insulinlike growth factor II mRNA binding protein 3 (IMP3) on biliary cytology specimens. Methods : We selected 40 patients who had undergone a biliary cytology examination and whose diagnoses were confirmed by biopsy. The IMP3 expression was evaluated by immunohistochemical staining in both the tissue and the cytology specimens. Their histologic diagnoses were cholangiocarcinoma (n = 34), high grade dysplasia (n = 2), low grade dysplasia (n = 1) and non-biliary tumor or no tumor (n = 3). Results : On the histology, all the cases of cholangiocarcinoma or high grade dysplasia were positive for IMP3 (36/36). The low grade dysplasia or the normal bile ducts in the other 4 cases were negative for IMP3 (0/4). Of the 36 cases diagnosed as cholangiocarcinoma or high grade dysplasia histologically, 29 cases were positive for IMP3 on the cytology (29/36; sensitivity, 80.6%). Of the 4 cases without cholangiocarcinoma or high grade dysplasia, 1 case was weakly positive for IMP3 on the cytology. The histologic diagnosis of this case was hepatocellular carcinoma. Except for this hepatocellular carcinoma case, the specificity of IMP3 on the cytology was 100%. Conclusions : IMP3 is a useful diagnostic marker for cholangiocarcinoma on cytology.

참고문헌 (Reference)

1 Hamilton SR, "World Health Organization classification of tumors: pathology and genetics of tumors of the digestive systems" IARC Press 158-214, 2000

2 Jeng YM, "RNA-binding protein insulin-like growth factor II mRNA-binding protein 3 expression promotes tumor invasion and predicts early recurrence and poor prognosis in hepatocellular carcinoma" 48 : 1118-1127, 2008

3 Vikesaa J, "RNA-binding IMPs promote cell adhesion and invadopodia formation" 25 : 1456-1468, 2006

4 Ligato S, "KOC(K homology domain containing protein overexpressed in cancer)and S100A4-protein immunoreactivity improves the diagnostic sensitivity of biliary brushing cytology for diagnosing pancreaticobiliary malignancies" 36 : 561-567, 2008

5 Schaeffer DF, "Insulin-like growth factor 2 mRNA binding protein 3(IGF2BP3)overexpression in pancreatic ductal adenocarcinoma correlates with poor survival" 10 : 59-, 2010

6 Ikeda K, "IMP3/L523S,a novel immunocytochemical marker that distinguishes benign and malignant cells:the expression profiles of IMP3/L523S in effusion cytology" 41 : 745-750, 2010

7 Li D, "IMP3 is a novel prognostic marker that correlates with colon cancer progression and pathogenesis" 16 : 3499-3506, 2009

8 Walter O, "IMP3 is a novel biomarker for triple negative invasive mammary carcinoma associated with a more aggressive phenotype" 40 : 1528-1533, 2009

9 Findeis-Hosey JJ, "IMP3 expression is correlated with histologic grade of lung adenocarcinoma" 41 : 477-484, 2010

10 Riener MO, "IMP3 expression in lesions of the biliary tract:a marker for high-grade dysplasia and an independent prognostic factor in bile duct carcinomas" 40 : 1377-1383, 2009

11 Noske A, "IMP3 expression in human ovarian cancer is associated with improved survival" 28 : 203-210, 2009

12 Mueller-Pillasch F, "Expression of the highly conserved RNA binding protein KOC in embryogenesis" 88 : 95-99, 1999

13 Li L, "Expression of RNA-binding protein IMP3(KOC)in benign urothelium and urothelial tumors" 39 : 1205-1211, 2008

14 Li KH, "Expression of IMP3 in osteosarcoma and its clinical significance" 25 : 426-427, 2009

15 Mentrikoski MJ, "Diagnostic utility of IMP3 in segregating metastatic melanoma from benign nevi in lymph nodes" 22 : 1582-1587, 2009

16 Jiang Z, "Combination of quantitative IMP3 and tumor stage:a new system to predict metastasis for patients with localized renal cell carcinomas" 14 : 5579-5584, 2008

17 Lu D, "An oncofetal protein IMP3:a new molecular marker for the detection of esophageal adenocarcinoma and high-grade dysplasia" 33 : 521-525, 2009

18 Nielsen J, "A family of insulin-like growth factor II mRNAbinding proteins represses translation in late development" 19 : 1262-1270, 1999

19 Nielsen FC, "A family of IGF-II mRNA binding proteins(IMP)involved in RNA trafficking" 234 : 93-99, 2001