The method and medium for obtaining callus and maintaining growth in tissue culture of koraiensis, P. rigida, P densiflora and P. thunbergii were adopted and the results are summarized as follows :
1. When cultivated in the Whites medium with each of...
The method and medium for obtaining callus and maintaining growth in tissue culture of koraiensis, P. rigida, P densiflora and P. thunbergii were adopted and the results are summarized as follows :
1. When cultivated in the Whites medium with each of seed extracted natural media, P. koraiensis only formed callus but P. rigida, P. densiflora and P,. thunbergii formed none.
2. When P. koraiensis was cultivated in a Whites medium modified by chestnut seed extract, firm brown callus was initiated at an explanat of it and has been successfully growing for 5 months.3. As for Korean pine seed extract, a higher concentration (75~90 mg/ℓ) inhibited the callus formation at an explant of P. koraiensis more than a lower concentration (15~30 mg/ℓ) but as for the chestnut extract, the opposited was the case.
4. The longer the size of explants on the culturing medium was, the less callus formation was made.