Effects of biphasic calcium phosphate on bone formation in human fetal osteoblasts = Biphasic Calcium Phosphate가 태아골모세포의 골 형성에 미치는 영향

국문 초록 (Abstract)

목적 : 이 연구의 목적은 치과 영역에서 골 재생을 촉진하기 위해, 현재 많이 사용하고 있는 BDX(bovinederived xenograft)와 비교하여 BCP(biphasic calcium phosphate)의 효과를 알아보기 위함이다. 실험 재료 및 방법: 본 연구는 태아골모세포주(hFOB 1.19)를 사용하였으며, 사용된 골 이식재에 따라 2개의 실험군으로 구분하였고, 각 실험에 적절한 농도의 BDX와 BCP를 첨가하였다. 그리고, 세포 증식도 검사, 교원질 합성량 분석, 염기성 인산분해효소 활성도 측정, Western blot 분석을 통한 OC과 BSP의 발현 정도등의 실험을 진행하였다. 결과 : BDX와 BCP는 대조군과 비교하여 세포 증식에서 유의한 차이가 없었지만, 교원질 합성량, 염기성 인산분해효소의 활성, 그리고 OC과 BSP의 발현에 있어 대조군과 비교하여 유의하게 증가를 보였다. 그러나, 두 이식재간의 유의한 차이는 보이지 않았다. 결론 : 본 실험실적 연구에서 BCP는 골모세포분화에 긍정적인 영향을 미침으로써 효과적인 이식재로 사용할 수 있음을 가늠할수 있었다.

참고문헌 (Reference)

1 "The osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen which promoted cell-attachment of osteoblasts" 97-102, 1996

2 "The osteoblast:a sophisticated fibroblast under central surveillance" 289 : 1501-4, 2000

3 "The effects of calcium phosphate particles on the growth of osteoblasts" 37 : 324-34, 1997

4 "S100A4:a novel negative regulator of mineralization and osteoblast differentiation" 18 : 493-501, 2003

5 "Qualitative and quantitative comparative study on different filling materials used in bone tissue regeneration:a controlled clinical study" 14 : -215, 1994

6 "Protein-chemical analysis of Bio-Oss bone substitute and evidence on its carbonate content" 22 : 1005-1012, 2001

7 "Osteogenic potential in vitro of human bone marrow cells cultured on macroporous biphasic calcium phosphate ceramic" 44 : 98-108, 1999

8 "Macroporous biphasic calcium phosphate ceramics:influence of macropore diameter and macroporosity percentage on bone ingrowth" 19 : 133-139, 1998

9 "Hydroxyapatite coated with heaptocyte growth factor(HGF)stimulates human osteoblasts in vitro" 82 : 457-60, 2000

10 "Evaluation of ceramics composed of different hydroxyapatite to tricalcium phosphate ratios as carriers for rhBMP-2" 22 : 1643-51, 2001

11 "Efficacy of porous bovine bone mineral in various types of osseous deficiencies:clinical observations and literature review" 21 : 395-405, 2001

12 "Effects of tricalcium phosphate bone graft materials on primary cultures of osteoblast cells in vitro" 15 : 119-125, 2004

13 "Effects of sintered bovine bone on cell proliferation and osteoblastic expression in MC3T3-E1 osteoblast-like cells" 586-92, 1999

14 "Effects of orthovanadate on collagen and fibronectin synthesis and alkaline phosphatase activity in MC3E3-E1 osteoblast cells J of Korean Orthop Assoc 2003" 133-41, 2003

15 "Effects of alpha-TCP and TetCP on MC3T3-E1 proliferation" naka (naka): 831-6, 2003

16 "Effects of Laddec on the formation of calcified bone matrix in rat calvariae cells culture" 20 : 1155-66, 1999

17 "Development and characterization of a conditionally immortalized human fetal osteoblastic cell line" 10 : 178-86, 1995

18 "Development and characterization of a conditionally immortalized human fetal osteoblastic cell line" 10 : 178-86, 1995

19 "Clinical and histologic observations of sites implanted with intraoral autologous bone grafts or allografts.15 human case reports" 67 : 1025-33, 1996

20 "Bone sialoprotein(BSP)is a crucial factor for the expression of osteoblastic phenotypes of bone marrow cells cultured on type I collagen matrix" 66 : 388-396, 2000

21 "Biomaterials in orthopaedic surgery:effects of different hydroxyapatites and demineralized bone matrix on proliferation rate and bone matrix synthesis by human osteoblasts" 16 : 397-402, 1995

22 "Anorganic bovine bone supports osteoblastic cell attachment and proliferation" 70 : 364-9, 1999

23 "A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics" 20 : 1799-806, 1999