An ammonium sulfate sensitive chitinase from Streptomyces sp. CS501

다국어 초록 (Multilingual Abstract)

A chitinase from Streptomyces sp. CS501 wasisolated from the Korean soil sample, purified by singlestepchromatography, and biochemically characterized.
The extracellular chitinase (Ch501) was purified to 4.60fold with yield of 28.74 % using Sepharose Cl-6B column.
The molecular mass of Ch501 was approximately 43 kDaas estimated by SDS-PAGE and zymography. The enzyme(Ch501) was found to be stable over a broad pH range(5.0–10.0) and temperature (up to 50 C), and have anoptimum temperature of 60 C. N-terminal sequence ofCh501 was AAYDDAAAAA. Intriguingly, Ch501 washighly sensitive to ammonium sulfate but it’s completelysuppressed activity was recovered after desalting out. TLCanalysis of Ch501 showed the production of N-acetyl Dglucosamine(GlcNAc) and Diacetylchitobiose (GlcNAc)2,as a principal hydrolyzed product. Ch501 shows antifungalactivity against Fusarium solani and Aspergillus brasiliensis,which can be used for the biological control offungus. As has been simple in purification, stable in a broadrange of pH, ability to produce oligosaccharides, andantifungal activity showed that Ch501 has potential applicationsin industries as for chitooligosaccharides productionused as prebiotics and/or for the biological control ofplant pathogens in agriculture.

참고문헌 (Reference)

1 Miller, G.L., "Use of dinitrosalicylic acid reagent for determination of reducing sugar" 31 : 426-428, 1959

2 Ordentlich, "The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii" 78 : 84-88, 1988

3 Ober, C., "The chitinase and chitinase-like proteins : A review of genetic and functional studies in asthma and immune-mediated diseases" 9 : 401-408, 2009

4 Mukherjee, G., "Purification, characterization, and antifungal activity of chitinase from Streptomyces venezuelae P10" 53 : 265-269, 2006

5 Rabeeth, M., "Purification of an antifungal endochitinase from a potential biocontrol agent Streptomyces griseus" 14 : 788-797, 2011

6 Okazaki, K., "Purification and properties of two chitinases from Streptomyces sp. J-13-3" 59 : 1586-1587, 1995

7 Yano, S., "Purification and characterization of chitinase A of Streptomyces cyaneus SP-27: An enzyme participates in protoplast formation from Schizophyllum commune mycelia" 72 : 54-61, 2008

8 Patil, N.S., "Purification and characterization of an extracellular antifungal chitinase from Penicillium ochrochloron MTCC 517 and its application in protoplast formation" 48 : 176-183, 2013

9 Nanjo, F., "Purification and characterization of an exo-beta-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis" 265 : 10088-10094, 1990

10 Dai, D.-H., "Purification and characterization of a novel extracellular chitinase from thermophilic Bacillus sp. Hu1" 10 : 2476-2485, 2011

11 Tanabe, T., "Purification and characterization of a 49 kDa chitinase from Streptomyces griseus HUT 6037" 89 : 27-32, 2000

12 김경자, "Purification and Characterization of Chitinase from Streptomyces sp. M-20" 한국생화학분자생물학회 36 (36): 185-189, 2003

13 Yan, Q., "Properties of a xylanase from Streptomyces matensis being suitable for xylooligosaccharides production" 58 : 72-77, 2009

14 Bhushan, B., "Production and characterization of a thermostable chitinase from a new alkalophilic Bacillus sp. BG-11" 88 : 800-808, 2000

15 Roberts, W.K., "Plant and bacterial chitinases differ inantifungal activity" 134 : 169-176, 1988

16 Christodoulou, E., "Overexpression, purification, and characterization of a thermostable chitinase(Chi40)from Streptomyces thermoviolaceus OPC-520" 23 : 97-105, 2001

17 Bhushan, B., "Isolation, purification and properties of a thermostable chitinase from an alkalophilic Bacillus sp. BG-11" 20 : 157-159, 1998

18 Xiayun, J., "Identification, characterization and functional analysis of a GH-18 chitinase from Streptomyces roseolus" 87 : 2409-2415, 2012

19 Vieille, C., "Hyperthermophilic enzymes : Sources, uses, and molecular mechanisms for thermostability" 65 : 1-43, 2001

20 Muzzarelli, R.A.A., "Current views on fungal chitin/chitosan, human chitinases, food preservation, glucans, pectins and inulin : A tribute to Henri Braconnot, precursor of the carbohydrate polymers science, on the chitin bicentennial" 87 : 995-1012, 2012

21 Laemmli, U.K., "Cleavage of structural proteins during the assembly of the head of bacteriophage T4" 227 : 680-685, 1970

22 Patil, R.S., "Chitinolytic enzymes : An exploration" 26 : 473-483, 2000

23 Dahiya, N., "Chitinase from Enterobacter sp. NRG4 : Its purification, characterization and reaction pattern" 8 : 14-25, 2005

24 Han, Y., "Characterization of antifungal chitinase from marine Streptomyces sp. DA11 associated with South China Sea sponge Craniella australiensis" 11 : 132-140, 2009

25 Bhattacharya, D., "Bacterial chitinases : Properties and potential" 27 : 21-28, 2007

26 Pradeep, G.C., "An extremely alkaline novel chitinase from Streptomyces sp. CS495" 49 : 223-229, 2013

27 Simkhada, J., "An ammonium sulfate sensitive endoxylanase produced by Streptomyces" SPRINGER 36 (36): 819-825, 2013

28 Bradford, M.M., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" 72 : 248-254, 1976

29 Pradeep, G.C., "A novel thermostable cellulase free xylanase stable in broad range of pH from Streptomyces sp. CS428" Elsevier BV 48 (48): 1188-1196, 2013