Quercetin Supplement is Beneficial for Altering Lipid Metabolism and Antioxidant Enzyme Activities in the Middle of Ethanol Feeding in Rats

다국어 초록 (Multilingual Abstract)

The current study examined the effect of quercetin supplements on the lipid-lowering and antioxidant metabolism in ethanol-fed rats. The control group(E8) received ethanol only diet for 8 wks, whereas the other group(E8Q4) received a diet including quercetin supplementation(0.05%, wt/wt) for 4 wks while on the ethanol diet for 8wks. The hepatic alcohol dehydrogenase activity was significantly higher in the E8Q4 group than in the E8 group. Supplementation with quercetin significantly elevated the HDL- cholesterol concentration, the HDL-C/total-C ratio, and lowered the atherogenic index(AI) compared to the E8 group. The hepatic triglyceride and cholesterol contents were significantly lowered by the quercetin supplement compared to those of the control group. The hepatic HMG-CoA reductase and ACAT activities of the E8Q4 group were significantly lower than those of the E8 group. The overall potential for antioxidant defense was significantly enhanced by the quercetin supplement, as indicated by a decrease in plasma and hepatic TBARS levels. The hepatic GSH-Px and G6PD activities were significantly higher in the E8Q4 group compared to the E8 group. The current results suggest that dietary quercetin leads to the inhibition of HMG-CoA reductase and ACAT, which in turn lowers cholesterol levels and normalizes antioxidant enzyme activities.

참고문헌 (Reference)

1 "Tissue sulfhydryl group" 82 : 70-77, 1959

2 "The protective effect of flavonol quercetin against ultraviolet an induced oxidative stress in rats" 154 : 21-29, 2000

3 "The feeding of ethanol in liquid diets" 550-553, 1986

4 "The effect of age and sex on glutathione reductase and glutathione peroxidase activities and on aerobic glutathione oxidation in rat liver homogenates" 112 : 109-115, 1969

5 "Studies on the quantitative and qualitative characterization of erythrocytes glutathion peroxidase" 70 : 158-169, 1979

6 "Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A" 14 : 625-631, 1973

7 "Regulation of acyl-CoA cholesterol acyltransferase activity in normal and atherosclerotic rabbit aortas Role of a cholesterol substrate pool" 320-339, 1986

8 "Quercetin and rutin as inhihitors of azoxymethanol-induced colonic neoplasia" 12 : 1193-1196, 1991

9 "Protective effect of epicatechin and quercetin on lipid peroxidation in phospholipid bilayers" 278-284, 1994

10 "Plasma and hepatic cholesterol and hepatic activities of 3 hydroxy 3 methyl glutaryl CoA reductase and CoA" 129 : 1182-1185, 1999

11 "Oxidative stress and apoptosis in HIV infection a role for plant-derived metabolites with synergistic antioxidant activity" immu (immu): 209-213, 1994

12 "Nutritional and metabolic effects of alcoholism" 15 : 583-589, 1999

13 "Micro assay for 3-hydroxy-3-methylglutaryl CoA reductase in rat liver and in L-cell fibroblasts" 370 : 369-377, 1974

14 "Methods of enzymatic analysis" Academic Press 1974

15 "Method of Enzymatic Analysis" Academic Press 673-684, 1974

16 "Its regulation in vivo and some of properties in vitro" 930-941, 1980

17 "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase" 47 : 469-474, 1974

18 "Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic dietary additives Relationship to antioxidant activity and to iron ion-reducing ability" 1673-1680, 1991

19 "Inhibition of lipoxygenase dependent lipid peroxidation by quercetin" 24 (24): 1443-1446, 1995

20 "Influence on prolonged ethanol intake on the level and turnover of alcohol and aldehyde degydrogenase and glutathione" 23 : 12-14, 1995

21 "Hypocholesterolemic effect of hesperidin mediated by inhibition of 3 hydroxy 3 methylglutaryl coenzyme A reductase and acyl coenzyme A" 19 : 1245-1258, 1999

22 "Gastric Cytoprotection" Plenum Press 38-44, 1999

23 "Flavonoids as antioxidants determination of radical-scavenging efficiencies" 343-355, 1990

24 "Examination of coumarins flavonoids and polysaccharopeptide for antibacterial activity" 27 : 1237-1240, 1996

25 "Evidence that the initial up regulation of phosphatidylcholine biosynthesis in free cholesterol loaded macrophage is an adaptive response that prevents cholesterol induced cellular necrosis" 271 : 22773-22781, 1996

26 "Enzymatic determination of unbound D mannose in serum" 35 : 761-766, 1997

27 "Enzymatic determination of total serum cholesterol" 20 (20): 470-475, 1974

28 "Effect of various flavonoids on lysosomes subjected to an oxidative or an osmotic stress" 44 : 1243-1248, 1992

29 "Effect of alcohol on postprandial lipemia with and without preprandial exercise" 20 : 58-64, 2001

30 "Different form of rat liver aldehyde dehydrogenase and their subcellular distribution" 397 (397): 9-23, 1975

31 "Depressed hepatic glutathione and increased zinc conjugates in alcoholic liver disease evidence of lipid peroxidation" 585-9, 1983

32 "Chronic ethanol consumption affects glutathione status in rat liver" 128 : 758-763, 1998

33 "Chemoprevention of colon carcinogenesis by dietary curcumin a naturally occurring plant phenolic compound" 55 : 259-266, 1995

34 "Chemistry of the 2-thio-barbituric acid test for determination of oxidative rancidity in foods" 15 : 602-607, 1964

35 "Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation" 38 : 1763-1769, 1989

36 "Cell toxicity induced by inhibition of acyl coenzyme A" 270 : 5772-5778, 1995

37 "Biochemical factors in alcoholic liver disease" 13 : 136-153, 1993

38 "Association between polymorphisms of ethanol metabolizing enzymes and susceptibility to alcoholic cirrhosis in a Korean male population" 16 : 745-750, 2001

39 "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction" 95 : 351-358, 1979

40 "Anti oxidant mechanisms involved in gastroprotective effects of quercetin" 53 : 82-88, 1998

41 "A simple method for isolation and purification of total lipids from animal tissues" 226 : 497-509, 1957

42 "A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding" 72 : 248-254, 1979

43 "A peroxidase-coupled method for the colorimetric determination of serum triglycerides" 29 (29): 538-542, 1983

44 "A comprehensive evaluation of the heparin-manganease precipitation procedure for estimating a high density lipoprotein cholesterol" 19 (19): 65-76, 1978