1-Bromoacetylpyrene 誘導體化劑를 利用한 Carboxyl基 含有成分의 分析에 관한 硏究(Ⅲ) : 生藥成分의 HPLC에 依한 定量 Determination of Carboxylic acids in Crude drugs by HPLC = Studies on the HPLC Determination of Carboxyl Group Using 1-Bromoactylpyrene as a Pre-labelling Reagent(Ⅲ)

다국어 초록 (Multilingual Abstract)

A highly sensitive and selective fluoresence high-performance liquid chromatography using 1-bromoace-tylpyrene as a pre-labelling reagent was applied to the determination of cinnamic acid and glycyrrhetic acid at p mol level.
The optimum conditions for the derivatization such as concentrations of KOH, 18-crown-6, and 1-bromoacetylpyrene, reaction temperature and reaction time were investigated.
The derivatives were separated on a reversed phase column using the mixture of acetonitrile, methanol and water as the mobile phase.
The effluent was menitored by fluorometer. Linearities of calibration curve were obtained between 0.5 p mol and 2.0 p mol.
The detection limit of cinnamic acid and glycyrrhetic acid was 0.05 p mol in a 20 ㎕ of injection volume.