미생물학적 방법에 의한 어체내 잔류 항균물질의 계열별 동정시험 = Determination of several families of antibacterial agent residues in fish by disk assay

다국어 초록 (Multilingual Abstract)

The possibility of identification of families of antibacterial agent residues in fish tissue was studied by disk assay using three test organisms, Bacillus subtilis BGA, Micrococcus luteus ATCC 9341, and Bacillus cereus var. mycoides ATCC 11778. In the present method, a simple clean-up procedure was performed to obtain the aqueous solution from homogenized flounder muscle sample(10g) in Mcilvaine buffer. Then, aqueous solution was fractionated into A and B to be used in disk assay by choloroform and Sep-Pak C_(18) cartridge column after being defatted in hexane. The chloroform layer of fraction A was used for the analysis of macrolide antibiotics(ML), sulfa drugs(SA), chloramphenicol(CP), and quinolone antibiotics(QN). Adsorbed materials to Sep-Pak C_(18) of fraction B were also employed for the analysis of penicillins(PC), tetracyclines(TC), and nitrofuran derivatives(NF) Minimun-detectable concentrations by the present method were, 0.1㎍/g for oxytetracycline, tetracycline, doxycycline, spiramycin and ciprofloxacin, 0.025㎍/g for erythromycin and ampicillin, 1.0㎍/g for sodium nifurstyrenate and florfenical, 0.25㎍/g for sulfamonomethoxi e and sulfadimethoxine, 2.5㎍/g for oxolinic acid and flumequine, and 15㎍/g for piromidic acid, respectively. Three test organisms showed different sensitivity patterns for each family of antibacterial agent. Sensitivity patterns were B. cereus ＞ B. subtilis ＞ M. luteus for TC and NF, M. luteus ＞ B, subtilis ＞ B. cereus for ML and PC, B. cereus ＝ B. subtilis ＞ M. luteus for CP and QN, and B. subtilis ＞ B. cereus＝M. luteus for SA. The present method utilizing these characteristics could be useful as a routine screening test for the determination of family of antibacterial agent residues in fish tissue.