Extraction of Phenolic Compounds from grape Seed Using Supercritical $CO_2$ and Ethanol as a Co-solvent = 초임계 이산화탄소와 에탄올 보조용매를 이용한 포도씨로부터의 페놀성 화합물의 추출

다국어 초록 (Multilingual Abstract)

A supercritical fluid extraction was performed for the extraction of phenolics from grape seeds which up to now have been discarded. The optimum condition for extraction process was predicted through response surface methodology using central composit experimental design. The extraction amount of grape seed phenolics was increased by increasing extraction temperature, pressure, and concentration of co-solvent (ethanol). The optimum extraction conditions were 84.83$^{\circ}$C, 51.50MPa and 1.27% ethanol. The yield of phenolics using SFE was higher with 3 folds than ethanol and 4 folds than hexane but less than 80% methanol. In the respects of food poisoning, the approved solvents were restricted to ethanol and hexane. So, SFE for extraction of phenolics could be powerful alternative method for solvent extraction.