Interleukin-2가 Mouse 비장의 혈구전구체세포에 미치는 영향에 관한 전자현미경적 연구 = An Electron Microscopic Study on the Hemopoietic Stem Cells of Spleen in interleukin-2 Treated Mice

다국어 초록 (Multilingual Abstract)

Interleukin-2, T-cell growth factor, is a glycoprotein produced by the lymphocytes and it is widely used as an immunotherapeutic agent on the hepatoma and various carcinoma. Interleukin-2 mediate antitumor effect by stimulating the proliferation of helper and cytotoxic T-cells and increasing the generation of lymphokine activated killer cells. Thus interleukin-2 was administered to the cancer patients and experimental animals, it could also effect on the lymphoblasts and reticular cells in the spleen. In this experiment, the author pursued the effect of interleukin-2 on the ultrastructure of the reticular cell of the mouse spleen.
Albino mice, DDY strain, weighing 20gm were used as experimental animals. The experimental animals were killed at 3, 6, 12, 24 and 48 hours after administration of 2,000,000 unit/kg interleukin-2. The specimens obtained from the spleen were prefixed in 2% glutaraldehyde-2.5% paraformaldehyde solution and postfixed in the 1% osmic acid. After dehydration, the specimen were embedded in Epon 812 and then ultrathin sections(600-800A??) were made and stained with urarnyl acetate and lead nitrate. And these preparations were observed with JEM 100cx-II electron microscope.
The results were as follows.
1. Medium sized lymphocytes, large lymphocytes and reticular cells were increased in the white pulp of the 3, 6 and 12 hours interleukin-2 treated mouse spleen. A few rod shaped rough endoplasmic reticulum were observed and mitochondria and vesicles were abundant in its cytoplasm.
2. Small lymphocytes, medium sized lymphocytes, large lymphocytes and reticular cells were seen as regular shapes and their cytoplasmic organelles were normal in the white pulp of the spleen in 24 and 48 hour interleukin-2 treated Mice group.
Consequently, it is suggested that interleukin-2 would induce the development of the cytoplasmic organelles in the reticular cells but the cytoplasmic organelles in the reticular cells would return to its normal state as the time goes by.