Colorimetric and fluorescence analysis of percarbonate and oxygen bleach via perhydrolysis of resorufin ester dye

국문 초록 (Abstract)

본 연구에서는 레조루핀 에스터 기반의 프로브를 사용하여 과탄산염의 검출을 위한 간단한 신호화 방법을 개발하였다. 과탄산염은 산업, 환경 분야에서 널리 사용되는 중요한 산화제로 레조루핀 에스터 기반의 프로브는 산화제, 금속 이온 및 음이온의 존재 하에서 과탄산염으로부터 생성된 과산화수소에 의한 피발로일 작용기의 과가수분해를 통해 과탄산염에 대해 선택적이고 민감한 신호화를 보였다. 신호화의 여부를 확인하기 위해 확인한 에스터 기반 프로브 중 레조루핀 피발레이트는 자발적 과가수분해에 대한 안정성과 빠른 검출 속도의 조건을 고려하였을 때 최적의 프로브로 확인되었다. 이 프로브로 과탄산염을 10분 이내에 검출할 수 있었으며, 검출 한계는 0.45 μM 이었다. 또한 이 프로브는 사무용 스캐너를 사용하여 가정용 산소계 표백제에서의 분석으로 과탄산염의 농도를 정량적으로 측정할 수 있었다.

다국어 초록 (Multilingual Abstract)

This study developed a simple method for percarbonate detection using a resorufin-ester-based probe as a signaling switch, was developed. Percarbonate is an oxidant that is widely used in industrial processes. Probe 1 exhibited selective and sensitive signaling behavior for percarbonate via the hydrolysis of the pivaloyl group by hydrogen peroxide that was produced from percarbonate, in the presence of commonly encountered oxidants, metal ions and anions. Perhydrolysis of the acylated resorufin by hydrogen peroxide, reinstates the colorimetric and fluorometric responses of the parent fluorescent chromophore. Therefore, colorimetric and fluorometric analysis are possible with this probe. Among tested ester-based probes as signaling switches, pivalate was the optimal probe owing to its resistance to spontaneous perhydrolysis as well as its fast signaling speed under testing conditions. The absorbance of effect of pH and water contests were measured for probe 1–3, the optimized conditions were set. The reaction between probe 1 and percarbonate was saturated within 10 min, and the limit of detection for percarbonate detection was estimated to be 0.45 μM. The probe was applied to the analysis of percarbonate in domestic oxygen bleach using an office scanner as a field-oriented signal-capturing device.

목차 (Table of Contents)

• 1. Introduction 1
• 2. Experimental details 6
• 2.1 General 6
• 2.2 Preparation of resorufin-based probes (13) 7
• 2.3 Preparation of stock solutions 14
• 2.4 Detection limit of percarbonate 14
• 2.5 Evidence for percarbonate signaling process 15
• 2.6 Determination of percarbonate in commercial bleach using an office scanner 15
• 2.6.1 Preparation of sample solutions 16
• 2.6.2 Spike and found test (Recovery test) 16
• 2.6.3 Construction of calibration curve using an office scanner 17
• 3. Results and discussion 18
• 3.1 Design and testing of acylated resorufin-based probes 13 18
• 3.2 Fluorescence signaling of percarbonate 41
• 3.3 Application to bleach analysis 46
• 4. Conclusion 53
• References 54
• 국문초록 58