인체망막색소상피세포에서 헴산화효소-1의 발현에 대한 산화스트레스 및 항산화물질의 영향 = Effects of Oxidative Stress and Antioxidant on the Expression of Heme Oxygenase-1 in Human RPE

다국어 초록 (Multilingual Abstract)

Purpose: To evaluate the effects of oxidative stress and antioxidantson heme oxygenase-1 (HO-1) in cultured human retinal
pigment epithelial (RPE) cells.
Methods: Cultured RPE cells were challenged with different concentrations of hydrogen peroxide (H₂O₂), and the HO-1 mRNA
level was determined by RT-PCR after 24 hours and 48 hours of incubation independently. Additionally, the HO-1 mRNA level
was measured after preincubating RPE cells with N-acetylcystein (NAC) as the antioxidant for 30 minutes and then challenging
the cells with H₂O₂.
Results: The expression of the HO-1 mRNA level increased after H₂O₂ exposure, and this level was proportional to the increased
H₂O₂ concentration (p<0.05). Expression of the HO-1 mRNA level decreased when the RPE cells were preincubated with NAC
(p<0.05).
Conclusions: In human RPE cells, the HO-1 level increased in proportion to the degree of oxidative stress and decreased with
exposure to antioxidants. Expression of HO-1 may be helpful in preventing retinal disease, which occurs due to oxidative
stresses such as age-related macular degeneration.

국문 초록 (Abstract)

목적: 인체망막색소상피세포에 산화스트레스 및 항산화물질이 헴산화효소-1 (heme oxygenase-1, HO-1)의 발현에 미치는 영향을 알아
보고자 하였다.
대상과 방법: 배양한 망막색소상피세포를 과산화수소(hydrogen peroxide, H₂O₂) 농도를 달리하여 각각 24시간, 48시간 동안 노출시킨 후
역전사중합효소반응을 통해 HO-1의 발현 정도를 관찰하였다. 또한 망막색소상피세포에 항산화제인 N-acetylcystein (NAC)을 30분
동안 전 처치한 뒤 과산화수소에 노출시킨 후 HO-1의 발현 정도를 비교하였다.
결과: 과산화수소에 노출된 망막색소상피세포에서 HO-1의 발현이 증가하였으며, 이는 과산화수소의 농도와 비례하여 증가하였다
(p<0.05). NAC와 함께 H₂O₂에 노출된 경우에는 H₂O₂ 단독으로 노출시킨 경우보다 HO-1의 발현이 감소되었다(p<0.05).
결론: 망막색소상피세포에서 HO-1은 산화스트레스 정도에 비례하여 증가하고 항산화제에 의해 감소한다. HO-1은 연령관련황반변성
같은 산화스트레스로 유발되는 망막질환을 예방하는데 도움이 될 것으로 기대된다.

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