Human immunodeficiency virus : innovative techniques for isolation and identification

목차 (Table of Contents)

• CONTENTS
• Preface = ⅸ
• An Overview of HIV Infection / Melnick, J.L. ; Butel,J.S.(Houston,Tex.) = 1
• Isolation of Human Immunodeficiency Virus from Infected Tissue Fluids, Tissues, and Cells / Khan,N.C.(Rockville,Md.) = 9
• Ⅰ. Introduction = 9
• Ⅱ. Isolation of HIV from Various Tissue Fluids = 10
• Ⅲ. Isolation of HIV from Tissues = 16
• Ⅳ. Isolation of HIV from Cells = 20
• Ⅴ. Studies on Sequential HIV Isolates = 22
• Ⅵ. Conclusions = 23
• References = 24
• Detection of HIV-1 Nucleic Acid in Clinical Samples Using Target DNA Amplification and Nonisotopic Probes / Kelier,G.H. ; Manak,M.M.(Rockville,Md.) = 28
• Ⅰ. Introduction = 28
• Ⅱ. Conventional Virus and Antibody Assays as Used in AIDS Diagnosis = 29
• Ⅲ. Use of the Polymerase Chain Reaction (PCR) for the Detection of HIV-1 Infection = 32
• Ⅳ. Technical Details of the PCR = 38
• Ⅴ. Sandwich Hybridization Assay = 44
• Ⅵ. Clinical Applications = 51
• Ⅶ. Discussion = 56
• Acknowledgments = 57
• References = 57
• Levels of CD$$4^+$$ Lymphocytes, Neopterin, and $$\beta _2$$-Microglobulin Are Early Predictors of Acquired Immunodeficiency Syndrome / Kr$$\ddot a$$mer, A. ; Biggar, R.J. (Bethesda, Md.) ; Fuchs, D. (Innsbruck) ; Rosenberg,P.S. ; Gail, M.H. ; Yellin, F.J. (Bethesda, Md.) ; Wachter, H. (Innsbruck) ; Goedert,J.J. (Bethesda,Md.) = 61
• Ⅰ. Introduction = 61
• Ⅱ. Subjects and Methods = 62
• Ⅲ. Cumulative AIDS Risk = 64
• Ⅳ. Sequential Changes in Markers = 65
• Ⅴ. Prediction of AIDS = 67
• Ⅵ. Comments = 70
• References = 72
• Diagnostic Assays for Human Immunodeficiency Virus Infection and for Clinical Progression by Use of Synthetic p17 Peptide Epitopes / Naylor, P.H. ; Heithaus, A. ; Kale,A. ; Erdos, M.R, ; Kirkley, J. ; Goldstein, A.L.(Washingion,D.C.) = 74
• Ⅰ. Introduction = 74
• Ⅱ. HIV p17 as a Target for Diagnostic Methods = 75
• Ⅲ. Peptides and Sera = 76
• Ⅳ. ELISA and HIV p17 Antigen Assay Protocol = 78
• Ⅴ. Antibody and Antigen Studies = 79
• Ⅵ. Conclusions = 85
• Acknowledgments = 88
• References = 89
• Bioassays for the Detection of HIV-1 and Practical Applications / Felber, B.K. ; Ciminale, V. : Schwarz, S. ; Pavlakis,G.N, (Frederick, Md.) = 91
• Ⅰ. Structure and Function of the HIV-1 : Proteins = 92
• Ⅱ. Regulation of Expression of HIV-1 = 92
• Ⅲ. Quantitative Bioassay for HIV-1 Based on trans-Activation = 94
• Ⅳ. Application of the Bioassay to Measure the Effects of Drugs = 96
• Ⅴ. Generation of a Bioassay to Measure the Effects of Drugs Directed against env-CD4 Interaction = 98
• Ⅵ. Different HIV-1 Isolates Can Be Distinguished According to Target Cell Tropism in T-Cell and Monocyte Cell Lines = 99
• Ⅶ. Summary and Conclusions = 101
• Acknowledgments = 101
• References = 102
• Diagnosis and Monitoring of HIV-l and HIV-2 Infection / Devare, S.G. ; Desai, S.M. ; Dawson, G.J. ; Hampl, H. : Hunt, J.C. (Abbott Park,Ⅲ.) = 105
• Ⅰ. Introduction = 105
• Ⅱ. HIV-1 Antigen Assay = 106
• Ⅲ. HIV-1 Antibody Assay = 109
• Ⅳ. HIV-1／HIV-2 (Combo) Assay = 110
• Ⅴ. Discrimination of HIV-1 and HIV-2 Infection = 112
• Ⅵ. Conclusions = 117
• References = 118
• Isolation of HIV-1 and Titration of Infected Peripheral Blood Mononuclear Cells by Microculture / Dimitrov, D.H. ; Melnick, J.L. ; Hollinger, F.B. (Houston, Tex.) = 122
• Ⅰ. Introduction = 122
• Ⅱ. Peripheral Blood Mononuclear Cells = 123
• Ⅲ. Standard Culture versus Microculture = 123
• Ⅳ. Varying Concentrations of Donor and Patient Cells = 125
• Ⅴ. Advantage of Microculture for Isolation of HIV and for Titrating HIV-Posi-tive PBMCs = 127
• Ⅵ. Conclusions = 128
• References = 129