Continuous Culture System 을 이용한 제일위 미생물의 단백질 합성에 관한 연구(3) 에너지원으로 Starch 의 영향 = RUMEN MICROBIAL PROTEIN SYNTHESIS IN A CONTINUOUS CULTURE SYSTEM(3) STARCH AS AN ENERGY SOURCE

국문 초록 (Abstract)

本 硏究는 continuous culture system을 이용하여 제1위 微生物 成長과 蛋白質合成에 있어 dilution rate(0.02h^(-1)), 0.05h^(-1), 0.10h^(-1))와 窒素源(100% urea-N, 75% urea-N+25% amino acid-N)의 영향을 測定하기 위해 실시하였으며 얻어진 結果를 아래와 같이 要約하였다
1. Dilution rate가 0.02h^(-1)에서 0.05 및 0.10h^(-1)로 증가함에 따라 100㎖당 微生物成長量(DM)은 평판 29% 및 38%가 증가하였고 또 100% urea-N에서 보다 75% urea-N+2S% ammino acid-N에서 27% 더 높았다.
2. 24시간 동안의 總 微生物成長量은 dilution rate가 0.02^(-1)일때 보다 0.05 및 0.10h^(-1)에서 각각 155% 및 448%나 증가하였고 25%의 urea-N을 amino acid-N으로 代替했을때 100% 증가하였다.
3. 微生物 純蛋白質 生成量도 dilution rate가 증가함에 따라 많아졌다. 즉 dilutlon rate가 0.02h^(-1)에서 0.05 및 0.10h^(-1)로 증가하였을 때 100㎖당 純蛋白質 含量은 각각 36%와 45%, 그리고 24시간 생산량은 109%나 증가되었다.
4. 利用된 starch의 mole當 微生物成長量(DM)은 dilution rate의 增加에 따른 差異가 없이 평균 27.45∼29.80g이었다. 그러나 25% urea-저온 25% amino acid-N으로 대체했을때 24.0g에서 32.9%으로 37%나 증가하였다.
5. Viable bacterial count는 dilution rate가 증가함에 따라 증가되었으며 특히 amino acid-N 添加區가 월등히 높았다.
6. 培養液中의 pH는 dilution rate가 증가함에 따라 그리고 amino acid-N을 첨가함에 따라 다소 감소되었으나 정상적인 범위내에 있었다.
7. NH₃-N濃度도 dilutlon rate의 증가와 amino acid-N의 첨가에 따라 감소되었다.
8. 총 VFA생성량은 dilution rate가 증가함에 따라 증가되었고 특히 amino acid-N 첨가시에 더욱 많았다.
9. Y_(ATP)는 100% urea-N에서는 dilution rate 가 0.02, 0.05 그리고 0.10h^(-1)로 증가함에 따라 6.58, 7.23 그리고 8.17로 증가되었고 75% urea-N+25% amino acid-N에서는 각각 7.27, 8.44 그리고 8.89였다.

다국어 초록 (Multilingual Abstract)

A experiment using the continuous culture system was conducted to study the effects of varying dilution rates(0.02h^(-1), 0.05h^(-1), 0.10h^(-1)) and nitrogen sources(100% urea-N, 75% urea-N+25% amino acid-N) on rumen microbial growth and protein synthesis with starch as an energy source.
1. Microbial cell dry matter yields were higher average of 29% and 38% in the dilution rate of 0.05 and 0.10h^(-1) over 0.02h^(-2) and were also higher 22% in 75% urea-N+25% amino acid-N as compared with 100% urea-N.
2. Total microbial cell dry matter yields for 24hours were higher 155% and 448% in the dilution rate of 0.05 and 0.10h^(-1), respectively than 0.02h^(-1) and were higher 100% in the medium which replaced 25% urea-N with 25% amino acid-N.
3. Microbial true protein yields were also increased as increasing dilution rates and were increased as urea-N replaced with amino acid-N. That is microbial true protein in incubation medium(㎎/100㎖) were higher 36% and 45% and total microbial true protein yields for 24hours were 239 and 623%, respectively in the dilution rate of 0.05 and 0.10h^(-1) as compared with dilution rate of 0.02h^(-1). When 25% urea-N replaced with 25% amino acid-N, microbial true protein were higher 89% in 100㎖ of incubation medium and total microbial true protein yields for 24hours were 109% higher than 100% urea-N.
4. Microbial cell dry matter yields per mole of fermented starch were not different with various dilution rate ranging 27.45∼29.80 g/mole of starch, but there were improved from 240 g in 100 urea-N to 32.9 g in 75% urea-N+25% amino acid-N.
5. Viable bacterial count were increased by increasing dilution rates and replacing amino acid-N.
6. pH of effluents was decreased by increasing dilution rate and replacing urea-N with amino acid-N.
7. NH₃-N concentration in effluent was also decreased by increasing dilution rate and replacing urea-N with amino acid-N.
8. Total VFA productions were increased by increasing dilution rate and by replacing 25% urea-N with 25% amino acid-N.
9. Y_(ATP) were 0.58 at 0.02h^(-1) but increased 7.23 and 8.17 at 0.05 and 0.10h^(-1) with 100% urea-N, but with 75% urea-N+25% amino acid-N, Y_(ATP) were 7.27, 8.44 and 8.89 at dilution ratio of 0.02, 0.05, and 0.10h^(-1).