Purification and Characterization of Lysozyme from Filipino venus, Ruditapes philippinarum

다국어 초록 (Multilingual Abstract)

Lysozyme from Filipino venus (Ruditapes philippinarum) was purified by ion-exchange and gel filtration chromatography. The purification fold and yield were 3,402 and 32.4%, respectively. The molecular weight was determined to be 13.4 kDa by SDS-PAGE. The specific activity of lysozyme was 3.76×105 units/mg protein with Micrococcus lysodeikticus as a substrate. The optimum temperature and pH of lysozyme were 75oC and 5.5,respectively. Lysozyme activity was decreased with about 45% after heat treatment for 30 min at 80oC, and completely inactivated at 100oC. It was activated by NaCl (10-70 mM), MgCl2, and CaCl2 (2-5 mM) whereas it was inhibited by ZnCl2 (2-30 mM).

참고문헌 (Reference)

1 Jolles P, "What’s new in lysozyme research? Always a model system, today as yesterday" 63 : 165-189, 1984

2 Klomklao S, "Trypsins from yellowfin tuna (Thunnus albacores) spleen: Purification and characterization" 144 : 47-56, 2006

3 Weber K, "The reliability of molecular weight determinations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis" 244 : 4406-4412, 1969

4 Salton MRJ, "The properties of lysozyme and its action on microorganisms" 21 : 82-99, 1957

5 Myrenes B, "Recovery of lysozyme from scallop waste" 24 : 69-80, 1994

6 Thammasirirak S, "Purification, characterization, and comparison of reptile lysozymes" 143 : 209-217, 2006

7 Grinde B, "Purification and characterization of two lysozymes from rainbow trout (Salmo gairdneri)" 173 : 269-273, 1988

8 Miyauchi K, "Purification and characterization of lysozyme from purple Washington clam Saxidomus purpurata" 72 : 1300-1305, 2000

9 Xue QG, "Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea virginica)" 139 : 11-25, 2004

10 Lockey TD, "Purification and characterization of lysozyme from hemolymph of Heliothis virescens larvae" 220 : 502-508, 1996

11 Miyauchi K, "Purification and characterization of lysozyme from brackishwater clam Corbicula japonica" 66 : 275-281, 2000

12 Fujimoto S, "Protein purification, cDNA cloning, and gene expression of lysozyme from eri-silkworm, Samia Cynthia ricini" 128 : 709-718, 2001

13 Nilsen IW, "Protein purification and gene isolation of chlamysin, a cold active lysozyme-like enzyme with antibacterial activity" 464 : 153-158, 1999

14 Kornegay JR, "Molecular adaptation of a leaf-eating bird: Stomach lysozyme of the hoatzin" 11 : 921-928, 1994

15 Shugar D, "Measurement of lysozyme activity and the ultra violet inactivation of lysozyme" 8 : 302-309, 1952

16 Min S, "Listeria monocytogenes inhibition by whey protein films and coatings incorporating lysozyme" 68 : 2317-2325, 2005

17 Wang S, "Isolation of a novel leguminous lysozyme and study on the antifungal activity" 47 : 341-347, 2012

18 Mecitoglu C, "Incorporation of partially purified hen egg white lysozyme into zein films for antimicrobial food packaging" 39 : 12-21, 2006

19 Wang S, "First report of a novel plant lysozyme with both antifungal and antibacterial activities" 327 : 820-827, 2005

20 Minagawa S, "Expression of Japanese flounder cDNA in insect cells" 25 : 439-445, 2001

21 Datta S, "Control of Listeria monocytogenes and Salmonella anatum on the surface of smoked salmon coated with calcium alginate coating containing oyster lysozyme and nisin" 73 : M67-M71, 2008

22 Schoentgen F, "Complete amino acid sequence of ostrich egg-white lysozyme, a goose-type lysozyme" 123 : 489-497, 1982

23 Laemmli UK, "Cleavage of structural proteins during assembly of the head of bacteriophage T4" 227 : 680-685, 1970

24 McHenery JG, "Characterization of the lysozyme of Mytilus edulis (L)" 71 : 583-589, 1982

25 Hikima S, "Characterization and function of kuruma shrimp lysozyme possessing lytic activity against Vibrio species" 316 : 187-195, 2003

26 Lee JM, "Biochemical and antibacterial properties of lysozyme purified from the viscera of scallops (Patinopecten yessoensis)" 32 : 474-489, 2008

27 Ito Y, "Amino acid sequences of lysozymes newly purified from invertebrates imply wide distribution of a novel class in the lysozyme family" 259 : 456-461, 1999

28 Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" 72 : 248-254, 1976

29 Ibrahim HR, "A helix-loop-helix-peptide at the upper lip of the active site cleft of lysozyme confers potent antimicrobial activity with membrane permeabilization action" 276 : 43767-43774, 2001