LINC00657 knockdown suppresses hepatocellular carcinoma progression by sponging miR-424 to regulate PD-L1 expression

다국어 초록 (Multilingual Abstract)

Background Hepatocellular carcinoma (HCC) is the sixth most commonly diagnosed malignant tumor and the fourth leadingcause of cancer-related deaths worldwide. As a novel non-coding RNA, LINC00657 was firstly identified as an oncogenicrole in breast cancer. However, few research focus on the effect of LINC00657 on the progression of HCC.
Objectives The purpose of this study was to investigate the effect of LINC00657 on HCC tissues and cells, and furtherexplore the potential mechanism.
Methods We first measured the expression of LINC00657 in HCC tissues and cell lines using qRT-PCR. Next we establishedLINC00657 knockdown in HCC cells. CCK-8 assay, cell invasion assay, flow cytometry analysis, qRT-PCR and westernblotting were applied to assess the role of LINC00657 knockdown in the biological behavior of HCC cells. The bioinformaticsanalysis and the rescue experiment were devoted to the underlying mechanism.
Results LINC00657 was remarkably overexpressed in HCC tissues and cell lines, associated with poor prognosis. LINC00657knockdown repressed cell proliferation and invasion, promoted cell apoptosis of HCC cell lines. The bioinformatics analysisshowed LINC00657 sponged miR-424 as a ceRNA. Besides, PD-L1 mimic rescued the suppression of si-LINC00657 inthe biological behavior of HCC cells.
Conclusion In a word, we observed LINC00657 regulated PD-L1 expression by sponging miR-424, thus affecting the developmentsof hepatocellular carcinoma. These findings LINC00657 may provide new evidence for therapeutic application inhepatocellular carcinoma.

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