돼지에서 분리한 대장균의 항균제 감수성과 테트라사이클린 내성 유전자의 분포 = Antimicrobial Susceptibility and Distribution of Tetracycline Resistance Genes in Escherichia coli Collected from Swine.

다국어 초록 (Multilingual Abstract)

Background : The high frequency of antibiotic-resistant bacteria against tetracyclines which are most widely used as animal feed additives contribute to the treatment of animals. And these bacteria are able to spread into human and are getting more difficult to treat for bacterial infections. The aim of this work was to investigate susceptibility of antibiotics and to detect tetracycline resistant genes from swine.
Methods : Bacteria were collected by rectal swab of swine from livestock farmhouse. The MICs (minimal inhibitory concentration) of penicillin (Pc), ampicillin (Am), tetracycline (Te) and erythromycin (Em) were determined according to the broth microdilution methodology of the Clinical and Laboratory Standards Institute (CLSI). PCR screening was carried out to identify possible tet genes that contributed to tetracycline resistance.
Results : All of the 61 isolates were Escherichia coli (E. coli) and were examined for MICs. MIC of antibiotics for the isolates ranged from 32 to ≥256 μg/ml for Pc, 4 to ≥256 μg/ml for Am, 32 to ≥256 μg/ml for Te, and 8 to ≥256 μg/ml for Em. Of the 61 strains analysed by PCR for the presence of the tetracycline resistance genes [tetA, tetB, tetC, tetD, tetE, tetG, tetGG, tetK, tetL, tetM, tetO, tetS, tetA(P), tetQ, and tetX], the most common determinants were tet(A) (61/61, 100%) and tet(B) (13/61, 21.3%). Tet(M) (6/61, 9.8%) were also found. Thirteen strains contained tet(A) and tet(B) genes (21.3%), 4 strains contained tet(A) and tet(M) genes (6.6%), and 2 strains contained tet(A), tet(B), and tet(M) genes (3.3%).
Conclusion : All the isolates were highly resistant to Te (MIC, 32 - ≥256 μg/ml) and contained at least 1 of 15 tetracycline resistance genes. However, the presence of more than one resistance determinants did not lead to noticeably higher MICs.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 1) 균 분리와 동정
• 2) 항균제 감수성 검사
• 3) Polymerase chain reaction (PCR)
• 결 과
• 1) 균 분리와 항균제 감수성 검사
• 2) Tetracycline 내성 유전자 분포
• 고찰
• 결론
• 참고문헌

참고문헌 (Reference)

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