Effects of Omega-3-Rich Harp Seal Oil on the Production of Pro-Inflammatory Cytokines in Mouse Peritoneal Macrophages

다국어 초록 (Multilingual Abstract)

Omega-3, a polyunsaturated fatty acid, is an essential fatty acid necessary for human health, and it protects against cardiovascular disease, inflammation, autoimmune diseases, and cancer. In the present study, we investigated the effects of omega-3-rich harp seal oil (HSO) on the production of nitric oxide (NO) and cytokines, such as tumor necrosis factor (TNF)-α, interleukin-(IL)-1β, IL-6, and IL-12/IL-23 (p40) in peritoneal macrophages of mice. The culture supernatants of murine macrophages exposed to lipopolysaccharide (LPS), HSO, or HSO+LPS were harvested to assay IL-1β, TNF-α, IL-6, and IL-12/IL-23 (p40) cytokines and NO. TNF-α, IL-1β, and IL-12/IL-23 (p40) levels, except IL-6, were lower in the culture supernatants of mouse peritoneal macrophages exposed to LPS plus HSO than those of the groups exposed to LPS alone. These observations demonstrate that omega-3-rich harp seal oil downregulates the production of the pro-inflammatory cytokines such as IL-1β, TNF-α, and IL-12/IL-23 (p40). These results suggest that HSO could be potentially used as a preventive agent or as an adjunct in anti-inflammatory therapy, if more research results were accumulated.

참고문헌 (Reference)

1 Hughes DA, "n-3 polyunsaturated fatty acids inhibit the antigen-presenting function of human monocytes" 71 : 357S-360S, 2000

2 Calder PC., "n-3 Polyunsaturated fatty acids, inflammation, and inflammatory diseases" 83 : 1505S-1519S, 2006

3 Hernández-Rodríguez J, "Tissue production of pro-inflammatory cytokines(IL-1, TNF, and IL-6)correlates with the intensity of the systemic inflammatory response and with corticosteroid requirements in giant-cell arteritis" 43 : 294-301, 2004

4 Simopoulos AP, "The omega-6/omega-3 fatty acid ratio, genetic variation, and cardiovascular disease" 17 : 131-134, 2008

5 Block RC, "The effects of EPA+DHA and aspirin on inflammatory cytokines and angiogenesis factors" 2 : 14-19, 2012

6 Simopoulos AP, "Omega-3 fatty acids in inflammation and autoimmune diseases" 21 : 495-505, 2002

7 Cirino G, "Nitric oxide and inflammation" 5 : 115-119, 2006

8 Mackman N, "Lipopolysaccharide induction of gene expression in human monocytic cells" 21 : 247-251, 2000

9 Castell JV, "Interleukin-6 is the major regulator of acute phase protein synthesis in adult human hepatocytes" 242 : 237-239, 1989

10 Romani L, "Interleukin-12 in infectious diseases" 10 : 611-636, 1997

11 Trinchieri G, "Interleukin-12 : a pro-inflammatory cytokine with immuno-regulatory functions that bridge innate resistance and antigen-specific adaptive immunity" 13 : 251-276, 1995

12 Kato T, "Influence of omega-3 fatty acids on the growth of human colon carcinoma in nude mice" 187 : 169-177, 2002

13 Bhagat K, "Inflammatory cytokines impair endothelium-dependent dilatation in human veins in vivo" 96 : 3042-3047, 1997

14 Rockey DC, "Inducible nitric oxide synthase in rat hepatic lipocytes and the effect of nitric oxide on lipocyte contractility" 95 : 1199-1206, 1995

15 Scott P, "IL-12 : initiation cytokine for cell-mediated immunity" 260 : 496-497, 1993

16 Woods A, "Genetics of inflammation and risk of coronary artery disease : the central role of interleukin-6" 21 : 1574-1583, 2000

17 Dyerberg J, "Fatty acid composition of the plasma lipids in Greenland Eskimos" 28 : 958-966, 1975

18 Arrington JL, "Docosahexaenoic acid suppresses function of the CD28 costimulatory membrane receptor in primary murine and Jurkat T cells" 131 : 1147-1153, 2001

19 Thies F, "Dietary supplementation with eicosapentaenoic acid, but not with other long-chain n-3 or n-6 polyunsaturated fatty acids, decreases natural killer cell activity in healthy subjects aged ＞55 y" 73 : 539-548, 2001

20 Robinson DR, "Dietary marine lipids suppress continuous expression of interleukin-1gene transcription" 31 : S23-S31, 1996

21 Fritsche KL, "Consumption of eicosapentaenoic acid and docosahexaenoic acid impair murine interleukin-12 and interferon-production in vivo. J Infect Dis 182 : S54-61" 182 : S54-S61, 2000

22 Osuchowski MF, "Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality" 177 : 1967-1974, 2006

23 Ross R, "Atherosclerosis-an inflammatory disease" 340 : 115-126, 1999