밀 형질전환과 이를 활용한 최신 연구동향 = Current Research Trends of Wheat Transformation and Biotechnology

다국어 초록 (Multilingual Abstract)

Wheat is one of the world's top three crops and is an important staple crop, accounting for 20% of the nutrient calories consumed by the world's population. However, due to its complex heterogeneous hexaploid chromosomes and vast genome of approximately 16 Gb, compared to those of other crops, molecular biology and biotechnology studies on wheat are lacking. In recent years, wheat genome analysis has been performed using the latest next-generation sequencing technology so that useful genes can be easily obtained, and wheat biotechnology research is accelerating in various fields. In this review, wheat transformation, an indispensable technique for developing new functional biotech wheat by revealing the function of wheat genes, is described in detail. In addition, the latest research results for overcoming plant diseases, abiotic stresses, and wheat-related diseases that are difficult to solve by classical breeding through wheat transformation and biotechnology are described.

참고문헌 (Reference)

1 신상현, "국산밀에서 농업형질 및 종실 형태가 수발아에 미치는 영향" 한국육종학회 45 (45): 346-357, 2013

2 박정미, "국내 발생 밀 붉은곰팡이병에 대한 약제의 배지상의 효과 검정과 포장 방제 약제 선발" 한국식물병리학회 18 (18): 194-200, 2012

3 Gupta, P. K., "Wheat genomics: present status and future prospects" 2008 : 896451-, 2008

4 Mamun, M. A., "Wheat Gene TaATG8j Contributes to Stripe Rust Resistance" 19 (19): 1666-, 2018

5 Ishida, Y., "Wheat (Triticum aestivum L.) transformation using immature embryos" 1223 : 189-198, 2015

6 Zhu X., "WRKY transcription factors in wheat and their induction by biotic and abiotic stress" 31 (31): 1053-1067, 2013

7 Chinnusamy, V., "Understanding and improving salt tolerance in plants" 45 (45): 437-448, 2005

8 Altenbach, S. B., "Transformation of the US bread wheat ‘Butte 86’ and silencing of omega-5 gliadin genes" 2 (2): 66-73, 2011

9 Hiei, Y., "Transformation of rice mediated by Agrobacterium tumefaciens" 35 (35): 205-218, 1997

10 Altenbach, S. B., "Towards reducing the immunogenic potential of wheat flour: omega gliadins encoded by the D genome of hexaploid wheat may also harbor epitopes for the serious food allergy WDEIA" 18 : 291-, 2018

11 Statista, "Topic: Gluten-Free Foods Market"

12 Zimin, A. V., "The first near-complete assembly of the hexaploid bread wheat genome" 6 (6): gix097-, 2017

13 Sanford, J. C, "The biolistic process" 6 (6): 299-302, 1988

14 Xu, H., "The DENSE AND ERECT PANICLE 1(DEP1)gene offering the potential in the breeding of high-yielding rice" 66 (66): 659-667, 2016

15 Porter, J. R., "Temperatures and the growth and development of wheat: a review" 10 (10): 23-36, 1999

16 Barro, F., "Targeting of prolamins by RNAi in bread wheat: effectiveness of seven silencing‐fragment combinations for obtaining lines devoid of coeliac disease epitopes from highly immunogenic gliadins" 14 (14): 986-996, 2016

17 Mao, X., "TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis" 63 (63): 2933-2946, 2012

18 Wang, M., "TaCYP81D5, one member in a wheat cytochrome P450 gene cluster, confers salinity tolerance via reactive oxygen species scavenging" 18 (18): 791-804, 2020

19 Zaenen, I., "Supercoiled circular DNA in crown-gall inducing Agrobacterium strains" 86 (86): 109-127, 1974

20 Groos, C., "Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white× red grain bread-wheat cross" 104 (104): 39-47, 2002

21 Diah Rachmawati, "Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars" Japanese Society for Plant Cell and Molecular Biology 23 (23): 521-524, 2006

22 Fontaine, M. M., "State drought programs and plans: survey of the Western United States" 15 (15): 95-99, 2014

23 Christou, P., "Stable transformation of soybean callus by DNA coated particles" 87 (87): 671-674, 1988

24 Chilton, M. D., "Stable incorporation of plasmid DNA into higher plant cells : the molecular basis of crown gall tumorigenesis" 11 (11): 263-271, 1977

25 Klein, T. M., "Stable genetic transformation of intact Nicotiana cells by the particle bombardment process" 85 (85): 8502-8505, 1988

26 Zhang, Y., "Simultaneous modification of three homoeologs of TaEDR1by genome editing enhances powdery mildew resistance in wheat" 91 (91): 714-724, 2017

27 Gil-Humanes, J., "Silencing of γ-gliadins by RNA interference (RNAi) in bread wheat" 48 (48): 565-568, 2008

28 International Wheat Genome Sequencing Consortium (IWGSC), "Shifting the limits in wheat research and breeding using a fully annotated reference genome" 361 (361): eaar7191-, 2018

29 Cao, A., "Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat" 108 (108): 7727-7732, 2011

30 McMullen, M., "Scab of wheat and barley : A re-emerging disease of devastating impact" 81 (81): 1340-1348, 1997

31 Asseng, S., "Rising temperatures reduce global wheat production" 5 : 143-147, 2015

32 Christou, P, "Rice transformation: bombardment" 197-203, 1997

33 Hirochika, H., "Retrotransposons of rice involved in mutations induced by tissue culture" 93 (93): 7783-7788, 1996

34 Beddow, J. M., "Research investment implications of shifts in the global geography of wheat stripe rust" 1 (1): 15132-, 2015

35 Kalunke, R. M., "Reduction of Allergenic Potential in Bread Wheat RNAi Transgenic Lines Silenced for CM3, CM16and 0.28 ATI Genes" 21 (21): 5817-, 2020

36 Schroth, M. N., "Reduction in yield and vigor of grapevine caused by crown gall disease" 72 (72): 241-246, 1988

37 Altenbach, S. B., "Reducing the immunogenic potential of wheat flour:silencing of alpha gliadin genes in a US Wheat cultivar" 11 : 20-, 2020

38 Menardo, F., "Reconstructing the Evolutionary History of Powdery Mildew Lineages (Blumeria graminis) at Different Evolutionary Time Scales with NGS Data" 9 (9): 446-456, 2017

39 Becker, D., "Protein composition and techno-functional properties of transgenic wheat with reduced α-gliadin content obtained by RNA interference" 85 (85): 23-, 2012

40 Acquaah, G, "Principles of Plant Genetics and Breeding" Blackwell 385-, 2007

41 Grunberg, A. M., "Pre-harvest sprouting in soft red winter wheat (Triticum aestivum L.) grown in the US mid-Atlantic" 30 (30): 95-102, 2002

42 Zang, X., "Overexpression of wheat ferritin gene TaFER-5B enhances tolerance to heat stress and other abiotic stresses associated with the ROS scavenging" 17 (17): 14-, 2017

43 Zang, X., "Overexpression of the wheat (Triticum aestivum L.) TaPEPKR2 gene enhances heat and dehydration tolerance in both wheat and Arabidopsis" 9 : 1710-, 2018

44 Mackintosh, C. A., "Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight" 26 (26): 479-488, 2007

45 Gao, H., "Overexpression of a WRKY Transcription Factor TaWRKY2 Enhances Drought Stress Tolerance in Transgenic Wheat" 9 : 997-, 2018

46 Wang, G. P., "Overaccumulation of glycine betaine enhances tolerance to drought and heat stress in wheat leaves in the protection of photosynthesis" 48 (48): 117-126, 2010

47 Jouanin, A., "Outlook for coeliac disease patients: towards bread wheat with hypoimmunogenic gluten by gene editing of α-and γ-gliadin gene families" 19 (19): 333-, 2019

48 Ali, S., "Origin, migration routes and worldwide population genetic structure of the wheat yellow rust pathogen Puccinia striiformis f. sp. tritici" 10 : e1003903-, 2014

49 Duplessis, S., "Obligate biotrophy features unraveled by the genomic analysis of rust fungi" 108 (108): 9166-9171, 2011

50 Fasano, A., "Nonceliac gluten sensitivity" 148 (148): 1195-1204, 2015

51 Igbinedion, S. O., "Non-celiac gluten sensitivity: All wheat attack is not celiac" 23 (23): 7201-7210, 2017

52 Elli, L., "Non-celiac gluten sensitivity : Time for sifting the grain" 21 (21): 8221-8226, 2015

53 Sollid, L. M., "Nomenclature and listing of celiac disease relevant gluten T-cell epitopes restricted by HLA-DQ molecules" 64 (64): 455-460, 2012

54 Frye, C. A., "Negative regulation of defense responses in plants by a conserved MAPKK kinase" 98 (98): 373-378, 2001

55 Ryu, J. G., "Mycotoxins produced by Fusarium Isolates from Barley in Korea" 6 (6): 21-27, 1990

56 Johnston, S. A., "Mitochondrial transformation in yeast by bombardment with microprojectiles" 240 (240): 1538-1541, 1988

57 MAFRA, "Ministry of Agriculture, Food and Rural Affairs Statistical Yearbook"

58 Martin, C., "Micropycnometer measurement of single kernel density of healthy, sprouted, and scab damaged wheats" 75 (75): 177-180, 1998

59 Lunn, G., "Mechanisms leading to excess alpha-amylase activity in wheat(Triticum aestivum, L)grain in the UK" 33 (33): 313-329, 2001

60 Sánchez‐León, S., "Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9" 16 (16): 902-910, 2018

61 Van Larebeke, N., "Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability" 252 (252): 169-170, 1974

62 Lopatin, M. I, "Influence of bacterial root canter on the development of the cherry tree in the orchard" 18 : 167-173, 1939

63 Sivamani, E., "Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1gene" 155 (155): 1-9, 2000

64 Cummins, G. B., "Illustrated genera of rust fungi" APS Press 2003

65 Depicker, A., "Homologous sequences in different Ti plasmids are essential for oncogenicity" 275 (275): 150-152, 1978

66 Chilton, M. D., "Highly conserved DNA of Ti plasmids overlaps T-DNA maintained in plant tumors" 275 (275): 147-149, 1978

67 Vasil, V., "Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus" 10 (10): 667-674, 1992

68 Farooq, M., "Heat stress in wheat during reproductive and grain-filling phases" 30 (30): 491-507, 2011

69 Scerf, K. A., "Gluten and wheat sensitivities-An overview" 67 : 2-11, 2016

70 Jiang, Y., "Genome-wide transcription factor gene prediction and their expressional tissue-specificities in maize" 54 (54): 616-630, 2012

71 Abe, F., "Genome-edited triple-recessive mutation alters seed dormancy in wheat" 28 (28): 1362-1369, 2019

72 Bliffeld, M., "Genetic engineering of wheat for increased resistance to powdery mildew disease" 98 : 1079-1086, 1999

73 Cheng, M., "Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens" 115 (115): 971-980, 1997

74 Jackson, J. F., "Genetic Transformation of Plants" Springer 202-, 2003

75 Sawahel, W. A., "Generation of transgenic wheat plants producing high levels of the osmoprotectant proline" 24 (24): 721-725, 2002

76 Toffaletti, D. L., "Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA" 175 (175): 1405-1411, 1993

77 Khatkar, B. S., "Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality" 35 (35): 299-306, 2002

78 Battais, F., "Food allergy to wheat:differences in immunoglobulin E-binding proteins as a function of age or symptoms" 42 (42): 109-117, 2005

79 Cheng, M., "Factors influencing Agrobacterium-mediated transformation of monocotyledonous species" 40 : 31-45, 2004

80 Fraley, R. T., "Expression of bacterial genes in plant cells" 80 (80): 4803-4807, 1983

81 Tian, B., "Expression of a rice soluble starch synthase gene in transgenic wheat improves the grain yield under heat stress conditions" 54 (54): 216-227, 2018

82 Kennedy, B. W, "Estimates of U.S. crop losses to prokaryote plant pathogens" 64 (64): 674-676, 1980

83 Xue, Z. Y., "Enhanced salt tolerance of transgenic wheat (Tritivum aestivum L.) expressing a vacuolar Na+/H+ antiporter gene with improved grain yields in saline soils in the field and a reduced level of leaf Na+" 167 (167): 849-859, 2004

84 Altenbach, S. B., "Elimination of omega-1, 2gliadins from bread wheat (Triticum aestivum) flour: effects on immunogenic potential and end-use quality" 10 : 580-, 2019

85 Bates, G. W, "Electroporation of plant protoplasts and tissues" 50 : 363-373, 1995

86 Hiei, Y., "Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA" 6 (6): 271-282, 1994

87 Zhang, Y., "Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA" 7 (7): 12617-, 2016

88 Ricker, A. J., "Effects of crown gall and hairy root on the growth of apple trees" 49 (49): 88-90, 1959

89 Gil-Humanes, J., "Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference" 107 (107): 17023-17028, 2010

90 Gupta, N. K., "Effect of short-term heat stress on growth, physiology and antioxidative defence system in wheat seedlings" 35 (35): 1837-1842, 2013

91 Nganje, W. E., "Economic impacts of fusarium head blight in wheat and barley: 1993-2001" North Dakota State University 53-, 2004

92 Dai, A, "Drought under global warming: a review" 2 (2): 45-65, 2011

93 Sanford, J. C., "Delivery of substances into cells and tissues using a particle bombardment process" 5 (5): 27-37, 1987

94 Tye-Din, J. A., "Comprehensive, quantitative mapping of T cell epitopes in gluten in celiac disease" 2 (2): 41ra51-, 2010

95 Makarevitch, I., "Complete sequence analysis of transgene loci from plants transformed via microprojectile bombardment" 52 (52): 421-432, 2003

96 Thakur, P., "Cold stress effects on reproductive development in grain crops: an overview" 67 (67): 429-443, 2010

97 Boynton, J. E., "Chloroplast transformation in Chlamydomonas with high velocity microprojectiles" 240 (240): 1524-1538, 1988

98 Wieser, H, "Chemistry of gluten proteins" 24 (24): 115-119, 2007

99 Chung, H. S, "Cereal scab causing mycotoxicosis in Korea and present status of mycotoxin researches" 3 (3): 31-36, 1975

100 Caio, G., "Celiac disease: a comprehensive current review" 17 : 142-, 2019

101 Leonard, M. M., "Celiac disease and nonceliac gluten sensitivity: A review" 318 (318): 647-656, 2017

102 Smith, F. D., "Biolistic transformation of prokaryotes : Factors that affect biolistic transformation of very small cells" 138 (138): 239-248, 1992

103 Johnston, S. A., "Biolistic transformation of animal tissue" 27 (27): 11-14, 1991

104 Sanford, J. C., "An improved helium driven biolistic device" 3 : 3-16, 1991

105 Hayta, S., "An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)" 15 (15): 121-, 2019

106 Gelvin, S. B, "Agrobacterium-mediated plant transformation:the biology behind the “gene-jockeying” tool" 67 (67): 16-37, 2003

107 Tripathi P., "A systems biology perspective on the role of WRKY transcription factors in drought responses in plants" 239 (239): 255-266, 2014

108 Figueroa, M., "A review of wheat diseases a field perspective" 19 (19): 99-111, 2018