To investigate the genetic polymorphism of serum protein of the horses on Cheju, we assigned horses for three populations, they were Cheju-native horses 40herds, which were precious natural monuments, Cheju-racing horses 55herds in Cheju Racing Associ...
To investigate the genetic polymorphism of serum protein of the horses on Cheju, we assigned horses for three populations, they were Cheju-native horses 40herds, which were precious natural monuments, Cheju-racing horses 55herds in Cheju Racing Association and Thoroughbred horses 46herds.
We analyzed the phenotypes and allelic frequencies of serum protein in three populations in Alb, Es, AlB, Tf loci by using a polyacrylamide gel electrophoresis.
In albumin locus, it was identified that it is controlled by 2 codominant autosommal alleles A and B, The phenotypes and allelic frequencies of serum protein were Alb^(A)=0.325, Alb^(B)=0.675 in Cheju-native horses, Alb^(A)=0.473, Alb^(B)=0.527 in Cheju-racing horses, Alb^(A)=0.139, Alb^(B)=0.860 in Thoroughbreds. In vitamin D bindmg protein locus, The phenotype of FF was observed in 100% in Cheju-native horses, 98.1% in Cheju-racing horses and 86.9% in Thoroughbreds. The phenotype of FS, SS was not observed in Cheju-native horses. Phenotype SS was not observed in all tested population. In Esterase locus, overall, the frequency of phenotype II was high. In Thoroughbreds, phenotype II was the highest in all tested populations. The gene frequencies of the separate populations were Es^(F)=0.337, Es^(1)=0.662, Es^(S)=0 in Cheju-native horses, Es^(F)=0.309, Es^(1)=0.581, Es^(S)=0.109 in Cheju-racing horses and Es^(F)=0.097, Es^(1)=0.815, Es^(S)=0.086 in Thoroughbreds. In glycoprotein locus, Phenotype KK was observed 100% in all tested populations. In transferrin locus, Tf^(F1) was nonexistent in Cheju-native horses, but in Thoroughbreds was the quantitative preponderance among all breeds. The frequencies of the separate alleles D, F1, F2, H2, 0 and R were 0.1, 0, 0.428, 0.014, 0.314 and 0.142 in Cheju-native horses, 0.16, 0.05, 0.41, 0.04, 0.06, 0.28 in Cheju-racing horses, and 0.365, 0.310, 0.229, 0.013, 0.081 and 0 in Thoroughbreds.