배양한 계배흉근의 근원세포의 증식과 분화에 대한 Chemically defined Media 의 효과 = Comparative Study of Chemically Defined media on the Proliferation and Differentiation of Chick Myoblasts in Culture

다국어 초록 (Multilingual Abstract)

Chemically defined media (CDM) are described that allow substantial proliferation and subsequent differentiation in primary cultures of cells derived from embryonic chick breast muscle. MCDB 201 (MCDB) and DME are found to be superior to RPMI 1640 (RPMI) as a basal medium, and MCDB specifically promotes cell proliferation whereas DME stimulates cell fusion. Ovotransferrin is found to be more effective than human transferrin and is effective only in Fe-complexed form but not in Fe-free form. The most suitable CDM appears to be that contains MCDB as a basal medium, as judged by criteria such as fusion index, cell proliferation, rate of DNA and protein synthesis, pattern of muscle-specific protein synthesis, and cell morphology.