만성 흡연에서 신경병증성 통증의 발현과 통증 조절 유전자의 발현 양상 = The Change of Neuropathic Pain and Pain Related Gene Expression Following Exposure to Chronic Smoking

다국어 초록 (Multilingual Abstract)

Background: Several studies have reported reduced pain and anxiety in smokers, and considerable evidence shows that smoking induces analgesia, which is thought to be nicotine-mediated. We investigated if smoking could reduce the development of neuropathic pain and nociceptive transmission in the spinal cord.
Methods: Sprague Dawley rats weighing 130-150 g were used for this experiment. The Animals were divided into two groups: the smoking group (S group) was exposed to cigarette smoking for 5 hours per day for 6 weeks at self-made smoking chamber: the control group (C group) was exposed to room air. After a 4-week exposure period, neuropathic pain was induced by left L5 spinal nerve ligation (SNL). Mechanical threshold and withdrawal response to 100% acetone were measured throughout the experiment. The changes in the expression of the c-fos and BDNF genes in the spinal cord were compared using real time PCR.
Results: Mechanical allodynia was induced after SNL in both groups, but no significant difference was observed between two groups. Cold allodynia after SNL was significantly less in S group than C group. In S group, the expression of c-fos was decreased at 5th day, but that of BDNF expression was significantly elevated at 5th day after SNL compared to C group.
Conclusions: Chronic exposure to cigarette smoke reduced the cold allodynia in neuropathic rats. The decreased expression of c-fos and elevated expression of BDNF in the spinal cord after SNL may contribute to antinociception.

국문 초록 (Abstract)

장기간의 흡연에 노출된 쥐에서 냉각 이질통의 발현은 둔화되어 나타났으며, 이는 흡연군에서 결찰 후 c-fos 발현의 감소 및 BDNF의 빠른 회복을 보인 것을 감안할 때 통증 전달 경로가 억제 되었음을 생각할 수 있다. 그러므로 신경병증성 통증을 유발시킨 후 행동학적 검사 및 분자생물학적 연구결과를 토대로 생각해 볼 때 만성적인 흡연은 신경병증성 통증의 발현을 억제와 관련된다고 할 수 있다.

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