Arachidonic acid 대사산물이 배양골조직의 골흡수에 미치는 영향에 관한 연구 = EFFECTS OF ARACHIDONIC ACID METABOLITES ON THE BONE RESORPTION IN VITRO

다국어 초록 (Multilingual Abstract)

This experiment was performed to study the arachidonic acid metabolites (prostaglandin E_2, leukotriene B_4 and HETE) and indomethacin of the bone resorption and the effect of indomethacin on the PGE_2-stimulated bone resorption in the organ culture.
Ulnae and radii or calvaria were removed from 19-day fetal rats prelabelled by subcutaneous injection of 200 μCi ^45CaCl_2 into mother rats at the 17th day of gestation. The control groups were cultured in fresh BGJb media, while experimental groups were cultured in media containing PGE_2 (10^-6M), indomethacin (10^-5M), leukotriene B_4 (0.1pM, 1pM, 10pM, 100pM and 1nM) or HETE (0.1pM, 1pM, 10pM, 100pM and 1nM) for 48 or 72 hours. Radioactivities of ^45Ca released into media and remained in bone after culture were respectively determined. The effects of each drugs were observed by the ratios of %-release of ^45Ca between control and experimental group.
The observed results were as follows.
1. PGE_2, at concentration of 10^-6M, increased ^45Ca release significantly after 24 and 48 hours of culture.
2. Indomethacin, at concentration of 10^-5M, decreased ^45Ca release after 24 and 48 hours of culture, but there was no significant difference.
3. LT and HETE, at all concentrations, had no effect on the bone resorption after 72 hours of culture.
4. Indomethacin, at concentration of 10^-5M, had no effect on the PGE_2-stimulated bone resorption after 72 hours of culture.

목차 (Table of Contents)

• I. 서 론
• II. 실험재료 및 방법
• III. 실험결과
• IV. 고 찰
• V. 결 론
• 참고문헌
• 영문초록