계작지모탕가미방(桂芍知母湯加味方)이 골관절염에 미치는 영향 = Effect of Kyejakjimo-tangkami (Guishaozhimu-tangjiawei) on Osteoarthritis

다국어 초록 (Multilingual Abstract)

ObjectivesThe purpose of this study is to prove the effect of Kyejakjimotangkami(KMK) on osteoarthritis.MethodsWe checked antioxidant activity and measured production of IL-1β, IL-6, TNF-α in RAW 264.7 cell after treat by KMK. Then we measured hind paw weight of Wister Rat with arthritis induced by MIA after KMK oral administration, checked Prostaglandin E2, IL-1β, IL-6, TNF-α, Osteocalcin, TIMP-1, MMP-9, LTB-4 in serum, ran histopathological test and μCT-arthrography.Results1. DPPH radical Scavenging was increased depend on concentration of KMK ethanol extract in RAW 264.7 cell. 2. Production of NO was significantly decreased by KMK ethanol extract on concentration of 200μg/ml in RAW 264.7 cell. 3. Production of IL-1β was significantly decreased by KMK ethanol extract on concentration of 200μg/ ml. And Production of IL-6 ,TNF-α were significantly decreased KMK ethanol extract of every concentration in RAW 264.7 cell. 4. Result of checking hind paw weight when ad-ministered KMK ethanol extract to Wister Rat with arthritis induced by MIA was sig-nificantly higher than control group and similar to normal group. 5. Production of Prostaglandin E2, IL-1β, Osteocalcin, TIMP-1, MMP-9 and LTB-4 in serum was sig-nificantly decreased by KMK ethanol extract after administerd to Wister Rat with arthritis induced by MIA. 6. In Hematoxylin & Eosin staining and Safranin-O staining, we could find inflammation of synovial cell, infiltration of macrophage and granulocyte and degeneration of cartilage and bone were decreased in comparison with control group. 7. When checked cartilage volume to examine degree of cartilage degeneration using μCT-arthrography, volume of cartilage was increased in comparison with control group.ConclusionsComparison of the results for this study showed that KMK ethanol extract have anti-inflammatory effectiveness and can protect cartilage and bone. So we expect that KMK can be used as a effective drugs for osteoarthritis. (J Korean Med Rehab 2013;23(4):39-57)