抗癌白朮散의 抗癌 및 抗轉移 效果에 關한 硏究 = Study on The Antitumor and Antimetastatic Effects of Hangambaekchulsan

국문 초록 (Abstract)

現在 大田大韓方病院에서 癌治療에 이용되고 있는 消積白朮散보다 有效한 抗癌 및 抗轉移 效果를 나타내며, 抗癌劑의 副作用을 抑制할 수 있는 새로운 韓方處方 開發을 위한 努力으로 抗暗性이 認定된는 4가지 藥物을 消積白朮散에 加味하여 抗癌 및 抗轉移 實驗을 施行하였다. in vitro에서 B16-Fo, A549 및 SUN-1등의 癌細胞에 대한 細胞毒性, A549 癌細胞에 대한 附着沮止效果, protein kinase C 沮害作用을 살펴보았고, in vivo에서는 S-180을 腹部로 移植한 후 生存比, 血液學的 變化를, B16-Fo 癌株를 尾靜脈에 移植하고 pulmonary colonization assay, 臟器의 病理組織變化 및 血液學的 變化 등을 檢討하였다. A549, B16-Fo, SNU-1, S-180 細胞에 대한 in vitro, in vivo 實驗에서 抗癌效果가 認定되었고, 癌株에 대하여서는 SNU-1, A549, B16-Fo 등의 順序로 特異한 抗腫瘍 效果를 보였으며, 抗轉利實驗으로 實施한 Protein Kinase C 活性沮害作用 및 動物組織檢事에서도 有效한 抗轉移 效果를 나타냈다.

다국어 초록 (Multilingual Abstract)

For the development of more effective new anti-cancer perscription, antitumor and antimetastatic effect of hangambaekchulsan(HBS) was evaluated experi-mentally and results were obtained as follows:
1. The concentration inhibiting cell growth below 55% was 10-3g/㎖ of HBSB against B16-Fo, 10-3g/㎖ of HBS and HBSB against A549 and 10-3g/㎖ of HBS, 10-³g/㎖∼10-⁴g/㎖ of HBSB against SNU-1.
2. HBS and HBSB acted specifically on the cancer cells of SNU-1, A549 and B16-Fo in order.
3. Medicinal herbs inhibiting cell growth such as B16-Fo, A549 and SNU-1 up over 30% were Orostachys Herba, Lonicerae Flos, Taraxaci Radix, Pinelliae Rhizoma, Scutellariae Radix, Curcumae Radix, Lithospermi Radix, Oldenlandiae diffusae Herba.
4. Over 50% inhibition of protein kinase C was shown in 125㎍/㎖ HBS and HBSB, Orostachys Herba, Lonicerae Flos, Lithospermi Radix, Coicis Semen, Aurantil nobilis Pericarpium.
5. Weight of B16-Fo tumor was significantly inhibited in HBS and HBSB treated groups as compared with control data and T/C % to S-180 was 136% and 147% respectively.
6. Number of platelet was significantly in HBS and HBSB treated groups, but platelet aggregation was significantly inhibited only in HBS treated group.
7. Over 50% inhibition of cell adhesion of A549 was 10-³g/㎖ of HBS and HBSB, Lonicerae Flos, Taraxaci Radix, Oldenlandiae diffusae Herba.
8. HBS and HBSB significantly inhibited pulmonary colonization as compared with control data.
9. In pathohistological changes HBS and HBSB inhibited the metastasis of B16-Fo as compared with control but HSBS was more effective.
From the above results HBS had antitumor and antimetastatic effects in dose-dependent manner, which suggests that HBS can be clinically applied to cancer patients.