Effect of Introducing Chitinase Gene on the Resistance of Tuber Mustard against White Mold

다국어 초록 (Multilingual Abstract)

The objective of this research was introduction of chit42 to tuber mustard plants through Agrobacterium- mediated transformation against white mold caused by Sclerotinia sclerotiorum. The binary plasmid pGisPEC1 was used in this study. Polymerase chain reaction analysis detected the transgene in 27 transformants with a transformation efficiency of 6.9%. Southern blot test was used to assess the copy number of transgene in tuber mustard plants. One, two, two, and two chit42- related bands were observed in the transformed lines TMB4, TMB7, TMB12, and TMB18, respectively. Enzymatic tests showed a significant increase in the activity of endochitinase in protein isolated from leaf tissues of chit42 transgenic 75-day tuber mustard lines. The pathogenicity of three pathogen isolates was tested on the leaves of transformed plans. The results of current study showed that expression of the gene chit42 in tuber mustard plants markedly reduced infection radius on the leaves 7 days after inoculation with the fungus.

참고문헌 (Reference)

1 Liu, H., "Transgenic Brassica napus L. lines carrying a two gene construct demonstrate enhanced resistance against Plutella xylostella and Sclerotinia sclerotiorum" 106 : 143-151, 2011

2 Duo-Chuan, L, "Review of fungal chitinases" 161 : 345-360, 2006

3 Punja Z. K, "Response of transgenic cucumber and carrot plants expressing different chitinase enzymes to inoculation with fungal pathogens" 80 : 999-1005, 1996

4 Ojaghian, M. R, "Potential of Trichoderma spp. and Talaromyces flavus for biological control of potato stem rot caused by Sclerotinia sclerotiorum" 39 : 185-193, 2011

5 Neuhaus, J. -M., "Pathogenesis-related proteins in plants" CRC Press 77-105, 1999

6 Roberti, R., "Induction of PR proteins and resistance by the biocontrol agent Clonostachys rosea in wheat plants infected with Fusarium culmorum" 175 : 339-347, 2008

7 Boland, G. J, "Index of plant hosts of Sclerotinia sclerotiorum" 16 : 93-108, 1994

8 Takahashi, W., "Increased resistance to crown rust disease in transgenic Italian ryegrass (Lolium multiflorum Lam.) expressing the rice chitinase gene" 23 : 811-818, 2005

9 Ojaghian, S., "Increased resistance against storage rot in transgenic carrots expressing chitinase chit42 from Trichoderma harzianum" 234 : 81-86, 2018

10 Deng, Z. N., "Identification of in vivo and in vitro lemon mutants by RAPD markers" 70 : 117-125, 1995

11 Roberti, R., "Evaluation of microbial products for the control of zucchini foot and root rot caused by Fusarium solani f. sp. cucurbitae race 1" 51 : 317-331, 2012

12 Nishizawa, Y., "Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase" 99 : 383-390, 1999

13 Zarinpanjeh, N., "Enhanced resistance to Sclerotinia sclerotiorum in Brassica napus by co-expression of defensin and chimeric chitinase genes" 57 : 417-425, 2016

14 Gentile, A., "Enhanced resistance to Phoma tracheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene" 126 : 146-151, 2007

15 Emani, C., "Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens" 1 : 321-336, 2003

16 Ojaghian, M. R., "Efficacy of UV-C radiation in inducing systemic acquired resistance against storage carrot rot caused by Sclerotinia sclerotiorum" 130 : 94-102, 2017

17 Baranski, R., "Chitinase CHIT36 from Trichoderma harzianum enhances resistance of transgenic carrot to fungal pathogens" 156 : 513-521, 2008

18 Ojaghian, M. R., "Brassica green manure rotation crops reduce potato stem rot caused by Sclerotinia sclerotium" 41 : 347-349, 2012

19 Mohammad Reza Ojaghian, "Biocontrol of Potato White Mold Using Coniothyrium minitans and Resistance of Potato Cultivars to Sclerotinia sclerotiorum" 한국식물병리학회 26 (26): 346-352, 2010

20 Ojaghian, M. R., "Application of acetyl salicylic acid and chemically different chitosans against storage carrot rot" 84 : 51-60, 2013

21 Qi, X. -H., "AFLP-based genetic diversity assessment among Chinese vegetable mustards (Brassica juncea (L.) Czern.)" 55 : 705-711, 2008

22 Murashige, T., "A revised medium for rapid growth and bio assays with tobacco tissue cultures" 15 : 473-497, 1962