브로콜리 잎 추출물의 항산화, 항균 및 항염증 활성 = Antioxidant, Antimicrobial and Anti-inflammatory Activities of Broccoli Leaf Extracts

다국어 초록 (Multilingual Abstract)

In order to explore the usability of non-edible leaf of broccoli and investigate the possibility of those leaves as a cosmeceuticals and nutricosmestics, in the present study, we investigated the yield, total polyphenol content, antioxidant, antimicrobial activity and anti-inflammatory activity of water and ethanol extracts of broccoli leaves. To measure the yield and total polyphenol content, the ethanolic extracts showed 1.24 times higher yield and 1.65 times larger in total polyphenol content than the water extracts. The results of antioxidation measurements showed that DPPH radical scavenging ability of water and ethanol extract were 55.56% and 94.75% at 2,500 μg/mL. In addition, the ABTS radical scavenging activity was 99.84% for ethanol extract concentration of 10,000 μg/mL. The antimicrobial activity against S. aureus, S. epidermidis, E. coli and P. acnes was not observed in the water extract, but the ethanol extract showed high antimicrobial activity. Especially, the antimicrobial activity against P. acnes was the highest and noteworthy. The cytotoxic concentrations of broccoli leaf extracts showed cell viability above 95% at concentrations 500 μg/mL and inhibition of NO production by 36.7% at 500 μg/mL for the ethanol extracts. Therefore, the ethanol extract of broccoli leaf can be used as a resource of natural cosmetic material for functional cosmeceuticals and nutricosmestics.

참고문헌 (Reference)

1 모정희, "화장품 소재로서 더덕과 발효더덕 추출물의 피부관련생리활성 비교탐색" 한국인체미용예술학회 14 (14): 49-60, 2013

2 김지영, "조리된 브로콜리의 항산화 효과 및 Sulforaphane 함량 분석" 동아시아식생활학회 19 (19): 564-569, 2009

3 정진아, "식용 및 약용 양치식물에서 부위별 추출물의 항산화활성 탐색" 한국자원식물학회 20 (20): 185-192, 2007

4 유민정, "상백피 추출물의 피부상재균에 대한 항균활성과 항산화 효과" 한국인체미용예술학회 11 (11): 87-98, 2010

5 김미경, "블루베리(Vaccinium ashei)잎 에탄올 추출물의 단회투여독성 및 피부 안전성 평가 연구" 한국인체미용예술학회 15 (15): 223-243, 2014

6 오유성, "브로콜리잎 분말 첨가 재래식 고추장의 이화학적 및 기능적 특성" 한국식품영양과학회 42 (42): 675-681, 2013

7 김대용, "브로콜리 추출물의 향장활성 효과" 한국인체미용예술학회 17 (17): 29-39, 2016

8 오재복, "브로콜리 줄기 분말을 혼합한 파운드 케익의 항산화 활성과 품질 특성에 미치는 영향" 한국식품영양학회 24 (24): 567-576, 2011

9 석대은, "브로콜리 용매추출물의 Bioactive Organosulfur 화합물의 분리 및 동정" 한국식품영양과학회 32 (32): 315-319, 2003

10 조경련, "브로콜리 분말을 첨가한 설기떡의 품질 특성" 한국식품영양학회 22 (22): 229-237, 2009

11 김미선, "브로콜리 꽃송이 및 줄기의 항산화, 항균 및 대장암 세포 생육억제효과" 한국미생물·생명공학회 42 (42): 58-66, 2014

12 성낙주, "머루 과피 용매추출물의 항산화성 및 아질산염 소거작용" 한국식품영양과학회 35 (35): 961-966, 2006

13 김미경, "망고 과육 및 껍질 추출물의 항산화 및 항균 활성 비교" 대한미용학회 13 (13): 113-118, 2017

14 정수지, "과채류 농축액의 항산화 및 상승효과" 한국식품과학회 47 (47): 240-245, 2015

15 박양원, "고온에서의 브로콜리 부위별 추출물의 항산화성 및 항균성" 한국식품영양과학회 34 (34): 759-764, 2005

16 Kim MR, "Volatile sulfur compounds, proximate components, minerals, vitamin C content and sensory characteristics of the juices of kale and broccoli leaves" 28 : 1201-1207, 1999

17 Fahey JW, "Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benwo[α]pyrene-induced stomach tumors" 99 : 7610-7615, 2002

18 Choi JH, "Studies on the antiaging action of Korean ginseng" 17 : 506-515, 1985

19 Arnao MB, "Some methodological problems in the determination of antioxidant activity using chromogen radicals : a practical case" 11 : 419-421, 2000

20 Pellegrini NRKe, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylene-benzothazoline-6- sulfonic acid radical cation decolorization assay" 299 : 379-389, 1999

21 Brody HJ, "Relevance of cosmeceuticals the dermatologic surgeon" 31 : 796-798, 2008

22 Brooks JD, "Potent induction of phase 2 enzymes in human prostate cells by sulforaphane" 10 : 949-954, 2001

23 Pande G, "Organic acids, antioxidant capacity, phenolic content and lipid characteriation of Georgia-grown underutilized fruit crops" 120 : 1067-1075, 2010

24 Raimer SS, "Managing pediatric atopic dermatitis" 39 : 1-14, 2000

25 Fenwick GR, "Glucosinoalated and their breakdown products in food and food plants" 18 : 123-201, 1983

26 Begona CA, "Fermented wheat germ extract inhibits glycolysis pentose cycle enzymes and induces apoptosis through poly(ADP-ribose) polymerase activation in Jurkat T cell leukemia tumor cells" 277 : 46408-46414, 2002

27 Verhoeven DT, "Epidemiological studies on Brassica vegetables and cancer risk" 5 : 733-748, 1996

28 Singleton VL, "Colorimetry of total phenolics with phosphomolybdic : phosphotungstic acid reagents" 16 : 144-158, 1965

29 Kurilich AC, "Carotene, tocopherol and ascorbate contents in subspecies of Brassica oleracea" 47 : 1576-1581, 1999

30 Van Poppel G, "Brassica vegetables and cancer prevention. Epidemiology and mechanisms" 472 : 159-168, 1999

31 Bailey AJ, "Biological significance of the intermolecular crosslinks of collagen" 251 : 105-109, 1974

32 Manach C, "Bioavailability and bioefficacy of polyphenols in humans" 81 : 230S-242S, 2005

33 Farag RS, "Antimicrobial activity of some Egyptian spice essential oils" 52 : 665-670, 1989

34 Jung EY, "Anti-oxidant oral cosmeceuticals" 14 : 37-41, 2009

35 Green LC, "Analysis of nitrate, nitrite and [15N]nitrate in biological fluids" 126 : 131-138, 1982

36 Aoshima H, "Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity" 52 : 5240-5244, 2004